Department of Hepatobiliary Surgery, Hainan Provincial People's Hospital, Haikou 570311, China.
Cell Biol Int. 2013 Feb;37(2):121-5. doi: 10.1002/cbin.10020.
To explore the effects of Mucins (MUC)1-shRNA on the proliferation and hypoxia inducible factor (HIF)-1alpha expression of human cholangiocarcinoma (CCA) QBC939 cells in vitro. MUC1-shRNA was constructed and transfected with Lipofectamine™ 2000 into cultured CCA cells. MUC1 mRNA and protein expression levels were determined by RT-PCR and Western blot, respectively. The cellular proliferation and HIF-1alpha expression of QBC939 cells were evaluated by the MTT assay and Western blot, respectively. After transfection, the expression levels of MUC1 mRNA and protein in the experimental group decreased significantly in QBC939 (P < 0.01). The proliferation of MUC1 shRNA-transfected group was 0.30 ± 0.05, 38.32 ± 1.43%, 15.18 ± 1.32%, and there were remarkable differences when compared with the control groups (P < 0.05). Significant inhibition of HIF-1alpha protein expression in MUC1 shRNA-transfected group was also discovered (P < 0.05). MUC1-shRNA could inhibit proliferation and significantly weaken HIF-1alpha protein expression of QBC939 cells, suggesting its potential as a therapeutic target of CCA.
探讨黏蛋白 1（MUC1）短发夹 RNA（shRNA）对体外人胆管癌细胞株 QBC939 增殖及缺氧诱导因子 1α（HIF-1α）表达的影响。
构建 MUC1-shRNA 并采用脂质体 2000 将其转染至培养的胆管癌细胞中。采用 RT-PCR 和 Western blot 分别检测 MUC1 mRNA 和蛋白的表达水平。采用 MTT 法和 Western blot 分别评估 QBC939 细胞的增殖和 HIF-1α表达。转染后，实验组中 MUC1 mRNA 和蛋白的表达水平在 QBC939 中显著降低（P＜0.01）。MUC1 shRNA 转染组的增殖率为 0.30±0.05、38.32±1.43%、15.18±1.32%，与对照组相比差异有统计学意义（P＜0.05）。MUC1 shRNA 转染组 HIF-1α 蛋白表达也明显受到抑制（P＜0.05）。
MUC1-shRNA 可抑制 QBC939 细胞的增殖，显著减弱 HIF-1α 蛋白的表达，提示其可能成为胆管癌的治疗靶点。
