Department of Physiology (Animal Physiology II), Faculty of Biology, Complutense University, Madrid 28040, Spain.
J Trace Elem Med Biol. 2013 Jul;27(3):236-41. doi: 10.1016/j.jtemb.2012.12.006. Epub 2013 Jan 20.
The aim of this study was to determine whether biochemical changes occurred in the liver and kidney of arsenic (As) exposed pups during gestation and lactation, and investigate the potential beneficial role of antioxidants against arsenic exposure damage. Pregnant wistar rats received the following treatments as drinking water: (1) distilled water; (2) arsenic (50 mg/L); (3) antioxidants: zinc (20 mg/L)+vitamin C (2 g/L)+vitamin E (500 mg/L); (4) arsenic (50 mg/L)+antioxidants. As- intoxicated pups showed significant decreases in liver cholesterol and triglyceride concentration, whereas Aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities were increased. Treatment with antioxidants returns these values to control ones. TBARS production in both organs and liver glutathione peroxidase (GPx) activity also increased whereas catalase (CAT) activity in both organs decreased in arsenic-exposed pups; the antioxidant administration only recover TBARS concentration to control values. Our findings suggest that administration of antioxidants during gestation and lactation could prevent some of the negative effects of arsenic.
本研究旨在确定孕期和哺乳期暴露于砷的幼鼠肝脏和肾脏是否发生生化变化，并探讨抗氧化剂对砷暴露损伤的潜在有益作用。怀孕的 Wistar 大鼠以饮用水的形式接受以下处理：（1）蒸馏水；（2）砷（50mg/L）；（3）抗氧化剂：锌（20mg/L）+维生素 C（2g/L）+维生素 E（500mg/L）；（4）砷（50mg/L）+抗氧化剂。砷中毒幼鼠肝脏胆固醇和甘油三酯浓度明显降低，而天门冬氨酸氨基转移酶（AST）和碱性磷酸酶（ALP）活性升高。抗氧化剂的处理使这些值恢复到对照值。两种器官的 TBARS 生成和肝脏谷胱甘肽过氧化物酶（GPx）活性也增加，而两种器官的过氧化氢酶（CAT）活性在砷暴露幼鼠中降低；抗氧化剂的给药仅使 TBARS 浓度恢复到对照值。我们的研究结果表明，在孕期和哺乳期给予抗氧化剂可能会预防一些砷的负面影响。
