Department of Biotechnology, University of Szeged, H-6726 Szeged, Közép fasor 52, Hungary.
Bioresour Technol. 2013 Mar;131:121-7. doi: 10.1016/j.biortech.2012.12.134. Epub 2012 Dec 27.
Terminal restriction fragment length polymorphism (T-RFLP) was applied to study the changes in the composition of the methanogens of biogas-producing microbial communities on adaptation to protein-rich monosubstrates such as casein and blood. Specially developed laboratory scale (5-L) continuously stirred tank reactors have been developed and used in these experiments. Sequencing of the appropriate T-RF fragments selected from a methanogen-specific (mcrA gene-based) library revealed that the methanogens responded to the unconventional substrates by changing the community structure. T-RFLP of the 16S rDNA gene confirmed the findings.
末端限制性片段长度多态性（T-RFLP）被应用于研究沼气产生微生物群落的产甲烷菌组成在适应富含蛋白质的单一底物（如酪蛋白和血液）时的变化。特别开发了实验室规模（5-L）连续搅拌罐反应器，并在这些实验中使用。从基于甲烷菌特异性（mcrA 基因）文库中选择的适当 T-RF 片段进行测序表明，产甲烷菌通过改变群落结构来响应非常规底物。16S rDNA 基因的 T-RFLP 证实了这一发现。
