用于厌氧消化池中产甲烷菌群快速分析的T-RFLP数据库。
A T-RFLP database for the rapid profiling of methanogenic communities in anaerobic digesters.
作者信息
Bühligen Franziska, Lucas Rico, Nikolausz Marcell, Kleinsteuber Sabine
机构信息
UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany.
UFZ - Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Permoserstr. 15, 04318 Leipzig, Germany.
出版信息
Anaerobe. 2016 Jun;39:114-6. doi: 10.1016/j.anaerobe.2016.03.013. Epub 2016 Apr 1.
We present a simple protocol for the cost- and time-efficient profiling of methanogens based on T-RFLP fingerprinting of mcrA amplicons. Sequence data were compiled from mesophilic lab-scale and full-scale biogas reactors operated under various conditions and fed with various substrates. The database facilitates the rapid identification of methanogens, thus reducing the need of cloning and sequencing.
我们提出了一种基于mcrA扩增子的T-RFLP指纹图谱对产甲烷菌进行经济高效且省时的分析方法。序列数据来自在不同条件下运行并投喂不同底物的中温实验室规模和全规模沼气反应器。该数据库有助于快速鉴定产甲烷菌，从而减少克隆和测序的需求。
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