Department of Pharmacy, First Affiliated Hospital of Fujian Medical University, Fuzhou, China.
Anal Bioanal Chem. 2013 Apr;405(10):3097-103. doi: 10.1007/s00216-013-6715-6. Epub 2013 Jan 23.
Human mammaglobin (MG) has been found to be the most specific molecular marker for the hematogenous spread of breast cancer cells. In our study, an electrochemical impedance spectroscopic DNA biosensor was established for the detection of MG in breast cancer patients. The working conditions for the biosensor, such as immobilization time, rinse process, and hybridization process, were optimized. Under the optimal conditions, the charge transfer resistance of the proposed DNA biosensor shows excellent correlation with the amount of the complementary oligonucleotides in the range from 1.0 × 10(-9) to 2.0 × 10(-8) M. The detection limit is 5.0 × 10(-10) M. The proposed biosensor was used to detect the polymerase chain reaction amplification products of actual clinical breast cancer samples. The results were compared with that obtained by conventional gel electrophoresis. The results indicate that the electrochemical impedance spectroscopic assay is significantly sensitive and time-saving. The simple strategy described here is expected to be used in clinical application for early diagnosis of breast cancer.
人乳球蛋白 (MG) 已被发现是乳腺癌细胞血行播散的最特异的分子标志物。在本研究中，我们建立了一种用于检测乳腺癌患者 MG 的电化学阻抗谱 DNA 生物传感器。对生物传感器的工作条件（如固定化时间、冲洗过程和杂交过程）进行了优化。在最佳条件下，所提出的 DNA 生物传感器的电荷转移电阻与互补寡核苷酸的量在 1.0×10(-9)至 2.0×10(-8) M 的范围内表现出极好的相关性。检测限为 5.0×10(-10) M。该生物传感器用于检测实际临床乳腺癌样本的聚合酶链反应扩增产物。将结果与常规凝胶电泳法进行比较。结果表明，电化学阻抗谱分析具有显著的灵敏度和省时的特点。这里描述的简单策略有望用于临床应用，以进行乳腺癌的早期诊断。
