快速鉴定蛋白质中的氢交换。

Rapid characterization of hydrogen exchange in proteins.

机构信息

NMR Research Centre, Indian Institute of Science, Bangalore-560012, India.

出版信息

Angew Chem Int Ed Engl. 2013 Feb 25;52(9):2440-3. doi: 10.1002/anie.201206828. Epub 2013 Jan 22.

DOI:10.1002/anie.201206828

Abstract

Kinetics and thermodynamics of amide hydrogen exchange in proteins can be investigated with two-dimensional (13)CO-(15)N NMR correlation experiments. The spectra are acquired with high resolution and sensitivity. A single type of experiment on one sample serves to characterize hydrogen-deuterium fractionation factors and hydrogen-exchange rates that span three orders of magnitude.

摘要

二维 (13)CO-(15)N NMR 相关实验可用于研究蛋白质中酰胺氢交换的动力学和热力学。该光谱具有高分辨率和灵敏度。在一个样品上进行单次实验即可用于表征跨越三个数量级的氢氘分馏因子和氢交换速率。