Department of Hepatobiliary Surgery, Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510630, China.
J Biochem. 2013 Apr;153(4):371-9. doi: 10.1093/jb/mvt003. Epub 2013 Jan 22.
α-Melanocyte-stimulating hormone (α-MSH) functions as a mediator of inflammation and immunity; however, the short half-life and high dose needed limit the comprehensive clinical application of α-MSH. The aim of this study was to generate human bone marrow-derived mesenchymal stem cells (MSCs) that express and secrete high levels of bioactive α-MSH. MSCs were obtained from a normal donor and assessed for proliferation, surface markers, and adipogenic and osteogenic differentiation. A lentivirus-encoding α-MSH was constructed. MSCs were infected with this lentivirus-encoding α-MSH and assessed for stability and the expression and secretion of bioactive α-MSH. The cumulative MSC expansion rates pre- and post-lentivirus infection were not significantly different (P > 0.05). The MSCs remained stable after infection with the lentivirus-encoding α-MSH. The concentration of α-MSH in the supernatants of MSCs infected with the lentivirus-encoding α-MSH was 17.55 ng/ml (P < 0.001), and a melanin assay indicated that bioactive α-MSH was secreted from MSCs infected with the lentivirus-encoding α-MSH, with an optical absorbance at OD(405) of 0.886 (P < 0.001). These results suggested that MSCs were promising cell carriers for the expression and secretion of high levels of bioactive α-MSH.
α-促黑素细胞激素（α-MSH）作为炎症和免疫的介质发挥作用；然而，半衰期短和所需的高剂量限制了 α-MSH 的全面临床应用。本研究的目的是生成表达和分泌高水平生物活性 α-MSH 的人骨髓间充质干细胞（MSCs）。从正常供体中获得 MSCs，并评估其增殖、表面标志物以及成脂和成骨分化。构建了编码 α-MSH 的慢病毒。用这种编码 α-MSH 的慢病毒感染 MSCs，并评估其稳定性以及生物活性 α-MSH 的表达和分泌。感染前和感染后 MSC 的累积扩增率没有显着差异（P > 0.05）。感染编码 α-MSH 的慢病毒后，MSCs 保持稳定。感染编码 α-MSH 的慢病毒的 MSC 上清液中 α-MSH 的浓度为 17.55 ng/ml（P < 0.001），并且黑色素测定表明，从感染编码 α-MSH 的慢病毒的 MSC 分泌了生物活性 α-MSH，在 OD（405）处的光吸收值为 0.886（P < 0.001）。这些结果表明 MSCs 是表达和分泌高水平生物活性 α-MSH 的有前途的细胞载体。
