Jewkes F E, McMaster D J, Napier W A, Houston I B, Postlethwaite R J
Royal Manchester Children's Hospital.
Arch Dis Child. 1990 Mar;65(3):286-9. doi: 10.1136/adc.65.3.286.
Sterile mid stream specimens of urine (MSSU) were obtained from 84 children in a hospital outpatient department. All 84 children collected urine at home by one of two Dipslide methods and by collection into boric acid within 24 hours of the hospital collected MSSU. The samples collected at home were posted to the hospital. Thirty six of the Dipslides (43%) and nine of the boric acid samples (10%) were not sterile but none had a pure growth of a single organism of greater than 10(5) organisms/ml. In addition, 17 of the Dipslides (20%) were returned with one or both media detached and therefore could not be relied upon to exclude urinary tract infection. In a second part to the study, 95 urines which showed a significant growth in primary culture were also cultured after storage in boric acid. Inhibition was noted in nine samples after storage in boric acid, seven of which were in underfilled bottles. Transport of specimens in boric acid produced less contamination than Dipslides but may inhibit growth in a small number of specimens. Technical failures with Dipslides were disappointingly high.
从一家医院门诊部的84名儿童中获取了中段无菌尿标本（MSSU）。所有84名儿童均在家中通过两种浸片法之一采集尿液，并在医院采集MSSU后24小时内将尿液收集到硼酸中。在家中采集的样本被邮寄到医院。36份浸片样本（43%）和9份硼酸样本（10%）并非无菌，但均无单一微生物的纯培养物数量超过10⁵个/ml。此外，17份浸片样本（20%）返回时一种或两种培养基脱落，因此无法依靠其排除尿路感染。在该研究的第二部分中，对95份在初次培养中显示有显著生长的尿液在储存在硼酸中后也进行了培养。在储存在硼酸中的9份样本中发现有抑制现象，其中7份来自未装满的瓶子。与浸片法相比，用硼酸运送标本产生的污染更少，但可能会抑制少数标本的生长。浸片法的技术失败率高得令人失望。
