硼酸对犬猫尿液细菌培养的影响。
The effect of boric acid on bacterial culture of canine and feline urine.
作者信息
Rowlands M, Blackwood L, Mas A, Cripps P, Crompton C, Burrow R
机构信息
Small Animal Teaching Hospital, University of Liverpool, Neston, Cheshire CH64 7TE.
出版信息
J Small Anim Pract. 2011 Oct;52(10):510-4. doi: 10.1111/j.1748-5827.2011.01102.x.
OBJECTIVE
To identify the optimal method of submission of canine and feline urine for bacterial culture.
METHODS
Cystocentesis samples from 250 animals (200 dogs, 50 cats) suspected of having urinary tract infections were collected. The reference aliquot, without preservative, was processed on site within 2 hours. Two further aliquots (one without preservative, one with boric acid) were stored at room temperature for up to 7 hours and then posted by guaranteed next day delivery to a commercial laboratory for analysis.
RESULTS
Forty-seven of the samples were positive on culture in the reference test. There was no significant difference between reference test results and those of samples posted without preservative (P=0·39), but samples posted in boric acid were significantly less likely to give a positive result (P=0·01). Samples posted without preservative had a sensitivity of 82% and a specificity of 98%; for boric acid, sensitivity was 73% and specificity 99%.
CLINICAL SIGNIFICANCE
Postal urine samples should be submitted to the laboratory in a plain sterile tube.
目的
确定犬猫尿液用于细菌培养的最佳送检方法。
方法
收集了250只疑似患有尿路感染的动物（200只犬、50只猫）的膀胱穿刺样本。未加防腐剂的参考等分样本在2小时内在现场进行处理。另外两份等分样本（一份未加防腐剂，一份加硼酸）在室温下保存长达7小时，然后通过次日必达快递寄送至商业实验室进行分析。
结果
参考检测中有47份样本培养呈阳性。参考检测结果与未加防腐剂送检样本的结果之间无显著差异（P = 0·39），但加硼酸送检的样本呈阳性结果的可能性显著降低（P = 0·01）。未加防腐剂送检的样本敏感性为82%，特异性为98%；加硼酸的样本，敏感性为73%，特异性为99%。
临床意义
邮寄尿液样本应使用普通无菌试管送至实验室。
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