Sidiakina T M, Ustiuzhanina S V, Novikova N D, Gorin S E, Esipova V V, Kalakutskiĭ L V
Antibiot Khimioter. 1990 Jan;35(1):11-4.
The influence of various preservation conditions of viability and antibiotic activity of Acremonium chrysogenum 281A and 305A strains producing cephalosporin C was studied. Cryogenization of the culture in the form of suspension of the vegetative mycelium in 20 per cent glycerol solution at a rate of 1 degree/min showed to be advantageous over lyophilization and L-drying. Cryogenization under such conditions provided rather high viability of the culture and preservation of its initial antibiotic activity for the period of its storage for at least 1.5 years in liquid nitrogen at a temperature of -196 degrees C.
研究了头孢菌素C产生菌产黄顶孢霉281A和305A菌株不同保存条件对其活力和抗生素活性的影响。将营养菌丝体悬浮于20%甘油溶液中，以1℃/分钟的速率进行冷冻，结果表明这种冷冻方法优于冻干法和L-干燥法。在这种条件下进行冷冻，可使培养物具有较高的活力，并在-196℃的液氮中保存至少1.5年期间保持其初始抗生素活性。
