Holecová Milada, Rozek Maria, Maryańska-Nadachowska Anna, Jánosková Viera
Department of Zoology, Comenius University, Mlynská dolina B-1, 842-15 Bratislava, Slovakia.
Folia Biol (Krakow). 2012;60(3-4):129-33. doi: 10.3409/fb60_3-4.129-133.
The karyotype structure of Protocalliphora falcozi is described for the first time. The diploid complement comprises 2n = 12, n(male) = 5+XY. Male mitotic plates include four pairs of long (metacentric) and one pair of medium-sized (submetacentric) autosomes. The submetacentric sex chromosomes X and Y are the smallest elements of the set. An achiasmatic meiosis was determined. The heterochromosomes do not form a heterovalent because they occur separately during the first meiotic division. The examined karyotype shows a pericentromeric position of constitutive heterochromatin in all autosomes. The longer arm of the X chromosome is heterochromatic, while the Y is entirely euchromatic. The NORs are active at mitotic prophase and at early meiotic stages. Small, bright signals were observed in the centromeric regions of autosomes after DAPI staining.
首次描述了法氏原丽蝇的核型结构。其二倍体染色体组包括2n = 12,n(雄性)= 5 + XY。雄性有丝分裂中期板包括四对长的(中着丝粒)和一对中等大小的(亚中着丝粒)常染色体。亚中着丝粒性染色体X和Y是该组中最小的元素。确定了无交叉减数分裂。异染色质不形成异价体,因为它们在第一次减数分裂期间分开出现。所检查的核型显示所有常染色体上组成型异染色质的着丝粒周围位置。X染色体的长臂是异染色质,而Y染色体完全是常染色质。核仁组织区在有丝分裂前期和减数分裂早期活跃。经DAPI染色后,在常染色体的着丝粒区域观察到小的明亮信号。