主要组织相容性复合体I类和II类在特发性炎性肌病中的表达
Major histocompatibility complex class I and II expression in idiopathic inflammatory myopathy.
作者信息
Das Leena, Blumbergs Peter C, Manavis Jim, Limaye Vidya S
机构信息
*Department of Rheumatology, North Manchester General Hospital, Pennine Acute Hospital NHS Trust, Manchester, UK †Department of Neuropathology, Hanson Institute, Institute of Medical and Veterinary Sciences, Adelaide ‡Department of Rheumatology, Royal Adelaide Hospital §Department of Medicine Adelaide University, Adelaide, Australia.
出版信息
Appl Immunohistochem Mol Morphol. 2013 Dec;21(6):539-42. doi: 10.1097/PAI.0b013e31827d7f16.
INTRODUCTION
We sought to study the intensity and pattern of major histocompatibility complex (MHC) I and II expression in muscle from patients with biopsy-proven idiopathic inflammatory myositis (IIM) including the subgroups, polymyositis (PM), dermatomyositis (DM), and inclusion body myositis (IBM).
METHODS
A total of 120 muscle biopsies (61 PM, 14 DM, and 45 IBM) were immunostained for MHC I and II. Staining was graded as follows. 0: no staining, 1+: ≤10% fibers, 2+: 10% to 25%, 3+: 25% to 50%, 4+: 50% to 99%, and 5+ 100%.
RESULTS
All IIM biopsies showed MHC I positivity; 93% showed MHC II positivity. The proportion of patients with MHC II score ≥3+ was higher in IBM than DM or PM. In DM, MHC I expression showed a perifascicular pattern. All IBM biopsies were immunopositive for MHC I and II; 30/45 were scored 5+.
DISCUSSION
Immunostaining for MHC I and II is a useful adjunctive test in diagnosis and subclassification of IIM.
引言
我们试图研究经活检证实的特发性炎性肌病（IIM）患者肌肉中主要组织相容性复合体（MHC）I和II的表达强度及模式，其中IIM包括多发性肌炎（PM）、皮肌炎（DM）和包涵体肌炎（IBM）亚组。
方法
对总共120份肌肉活检样本（61份PM、14份DM和45份IBM）进行MHC I和II免疫染色。染色分级如下：0级：无染色；1+级：≤10%的纤维染色；2+级：10%至25%；3+级：25%至50%；4+级：50%至99%；5+级：100%。
结果
所有IIM活检样本均显示MHC I阳性；93%显示MHC II阳性。IBM中MHC II评分≥3+的患者比例高于DM或PM。在DM中，MHC I表达呈束周模式。所有IBM活检样本的MHC I和II免疫染色均为阳性；45份中有30份评分为5+。
讨论
MHC I和II免疫染色是IIM诊断和亚分类的有用辅助检查。
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