Sparla A M, Overnell J
Dunstaffnage Marine Laboratory, Scotland, U.K.
Biochem J. 1990 Apr 15;267(2):539-40. doi: 10.1042/bj2670539.
Differential pulse polarography was used to monitor the titration of crab (Cancer pagurus) cadmium metallothionein with additional cadmium. Scatchard analysis was used to calculate an apparent second-order dissociation constant. A mean value of 3.60 x 10(-7) M was calculated at 20 degrees C in 0.016 M-NaClO4, pH 8.0.
采用差分脉冲极谱法监测蟹(黄道蟹)镉金属硫蛋白与额外镉的滴定过程。用Scatchard分析法计算表观二级解离常数。在20℃、0.016 M高氯酸钠、pH 8.0条件下,计算出的平均值为3.60×10⁻⁷ M。