1Department of Clinical Pharmacy and Pharmacy Management, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University Awka, Anambra State, Nigeria 2Department of Clinical Pharmacy and Pharmacy Management, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Nigeria 3Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria.
Am J Ther. 2013 Sep-Oct;20(5):507-13. doi: 10.1097/MJT.0b013e31820544be.
The study was carried out to determine the effect of ginger on the plasma pharmacokinetics of ciprofloxacin and Isoniazid in a rat model in phase 1. The effects of the herb on the penetration of ciproflacin and Isoniazid into the lung tissues were also determined in phase 2. In phase 1, Albino rats of both sexes (n = 20) were divided into 4 groups of 5 rats per group. Two groups received oral ciprofloxacin (20 mg/kg) and isoniazid (15 mg/kg). Other groups were fed with ginger (5 mg/kg) for 10 days followed by the drug administration on the 11th day. Blood samples were collected from each group at 0-, 0.5-, 1-, 2-, 5-, 8-, 12-, and 24-hour intervals. Plasma concentrations of the drugs were determined by a spectrophotometric method and the pharmacokinetic parameters determined using noncompartmental method as implemented in the winNonlin program. In phase 2, where the effects of the herb on the penetration of the drugs were determined, the concentrations of ciprofloxacin and isoniazid attained in the lung fluid of rats in the presence and absence of the herb were compared after a single oral dose of the drugs used in the same dose range as in phase 1. In the first phase, treatment with ginger significantly increased the area under the concentration-time curve of ciprofloxacin, whereas Vz and Cl were decreased. Ginger significantly decreased the area under the concentration-time curve of isoniazid, whereas Vz and Cl were increased. Ginger enhanced the penetration of ciprofloxacin and Isoniazid into the lung tissues; however, their rates of penetration were delayed.
这项研究旨在确定生姜对 I 期大鼠模型中环丙沙星和异烟肼血浆药代动力学的影响。在 II 期研究中，还确定了该草药对环丙沙星和异烟肼渗透到肺组织的影响。在 I 期研究中，将 20 只雄性和雌性白化大鼠（n = 20）分为 4 组，每组 5 只。两组大鼠分别口服环丙沙星（20 mg/kg）和异烟肼（15 mg/kg）。其他两组大鼠连续 10 天给予生姜（5 mg/kg），然后在第 11 天给药。在 0、0.5、1、2、5、8、12 和 24 小时时间点从每组收集血样。通过分光光度法测定药物的血浆浓度，并使用非房室模型方法（在 winNonlin 程序中实现）确定药代动力学参数。在 II 期研究中，评估了该草药对药物渗透的影响，比较了在单次口服相同剂量范围内的药物后，在存在和不存在草药的情况下，大鼠肺液中达到的环丙沙星和异烟肼的浓度。在第一阶段，与对照组相比，生姜治疗显著增加了环丙沙星的药时曲线下面积，而 Vz 和 Cl 则降低。生姜显著降低了异烟肼的药时曲线下面积，而 Vz 和 Cl 则增加。生姜增强了环丙沙星和异烟肼渗透到肺组织的能力；然而，它们的渗透速度被延迟。
