Shalitin C, Stanik V
Department of Biochemistry and Biophysics, Oregon State University, Corvallis 97331.
Biochem Biophys Res Commun. 1990 Apr 30;168(2):580-8. doi: 10.1016/0006-291x(90)92360-c.
Immunoblot analysis using antibody specific to yeast ras-related protein Yp20 revealed a 21kDa protein associated with HeLa cell chromatin which immunologically cross-reacted with this ras-related protein. The 21kDa protein can be rapidly released from the HeLa nuclei by micrococcal nuclease digestion. It seems to be associated with long mononucleosomes as determined by separation of chromatin subunits on sucrose gradients. We suggest that the 21kDa HeLa protein may be the human homologue of yeast Yp20.
使用针对酵母ras相关蛋白Yp20的特异性抗体进行免疫印迹分析，结果显示在HeLa细胞染色质中有一个与该ras相关蛋白发生免疫交叉反应的21kDa蛋白。该21kDa蛋白可通过微球菌核酸酶消化从HeLa细胞核中快速释放出来。通过蔗糖梯度分离染色质亚基确定，它似乎与长单核小体相关。我们认为，HeLa细胞中的21kDa蛋白可能是酵母Yp20的人类同源物。
