利用荧光和表面增强拉曼散射的单分子成像与光谱学
Single-molecule imaging and spectroscopy using fluorescence and surface-enhanced Raman scattering.
作者信息
Ishikawa M, Maruyama Y, Ye J-Y, Futamata M
机构信息
Joint Research Center for Atom Technology (JRCAT), 1-1-4 Higashi, Tsukuba, Ibaraki, 305-0046 Japan.
出版信息
J Biol Phys. 2002 Dec;28(4):573-85. doi: 10.1023/A:1021222318740.
We extended single molecule fluorescence imaging and time-resolved fluorometry from the green to the violet-excitation regime to find feasibility of detecting and identifying fluorescent analogs of nucleic-acid bases at the single-molecule level. Using violet excitation, we observed fluorescent spotsfrom single complexes composed of a nucleotide analogue and the Klenow fragmentof DNA polymerase I. Also, we implemented Raman imaging and spectroscopy of adenine molecules adsorbed on Ag colloidal nanoparticles to find feasibility of identifying nucleic-acid bases at the single-molecule level. Surface enhanced Raman scattering (SERS) of adenine molecules showed an intermittent on-and-off behavior called blinking. The observation of blinking provides substantial evidence for detecting single adenine molecules.
我们将单分子荧光成像和时间分辨荧光测定从绿色激发范围扩展到紫色激发范围，以探寻在单分子水平检测和识别核酸碱基荧光类似物的可行性。利用紫色激发，我们观察到了由核苷酸类似物与DNA聚合酶I的克列诺片段组成的单个复合物发出的荧光斑点。此外，我们还对吸附在银胶体纳米颗粒上的腺嘌呤分子进行了拉曼成像和光谱分析，以探寻在单分子水平识别核酸碱基的可行性。腺嘌呤分子的表面增强拉曼散射（SERS）呈现出一种称为闪烁的间歇性开关行为。闪烁现象的观察为检测单个腺嘌呤分子提供了重要证据。
Zotero 插件