Danev R, Okawara H, Usuda N, Kametani K, Nagayama K
Department of Physiological Sciences, the Graduate University of Advanced Studies, Okazaki, 444-8585 Japan.
J Biol Phys. 2002 Dec;28(4):627-35. doi: 10.1023/A:1021234621466.
We report a novel class of transmission electron microscope (TEM), the difference-contrast electron microscope (DTEM), which displays nanostructures of thin specimen objects in a topographical manner. Topography obtained by the difference-contrast develops shadowgraphs in pseudo three-dimension, namely volume-like representation of projected objects as if things are illuminated by light from one direction. The specific optical device tomanipulate electron waves for DTEM is the hemicircular π phase-plate, which appears to be quite distinguishable from the Zernike phase plate utilized in Zernike phase-contrast TEM, while both have to be placed onto the back-focal plane of the objective lens. The topographic images obtained with DTEM for ultrathin sections of kidney cells were compared with those obtained with conventional TEM. DTEM confirmed the experimental advantage of high contrast topography by visualizing ultrastructural details inside the cells.
我们报告了一种新型的透射电子显微镜（TEM），即差分对比电子显微镜（DTEM），它以形貌方式显示薄样品物体的纳米结构。通过差分对比获得的形貌以伪三维形式呈现阴影图，即投影物体的类似体积的表示，就好像物体是由来自一个方向的光照射一样。用于DTEM操纵电子波的特定光学装置是半圆形π相位板，它似乎与泽尼克相衬TEM中使用的泽尼克相位板有很大区别，而两者都必须放置在物镜的后焦平面上。将DTEM用于肾细胞超薄切片获得的形貌图像与传统TEM获得的图像进行了比较。DTEM通过可视化细胞内的超微结构细节，证实了高对比度形貌的实验优势。
