通过粘度测量法测定绵羊血清白蛋白溶液的一些流体动力学参数。
Determination of some hydrodynamic parameters of ovine serum albumin solutions using viscometric measurements.
作者信息
Monkos Karol
机构信息
Department of Biophysics, Silesian Medical Academy, H. Jordana 19, 41-808 Zabrze 8, Poland.
出版信息
J Biol Phys. 2005 May;31(2):219-32. doi: 10.1007/s10867-005-1830-z.
Abstract
The influence of protein concentration and temperature on the viscosity of ovine serum albumin (OSA) solutions was studied. The Mooney equation and a modified Arrhenius formula were used to described the viscosity-concentration and viscosity-temperature dependence of the solutions, respectively. The effective specific volume, the activation energy and entropy of viscous flow for hydrated OSA were calculated. The axial ratio and the dimensions of the main semi-axes of hydrated OSA were established. At low concentration limit, the temperature dependence of the intrinsic viscosity and Huggins coefficient is presented. Comparison of some hydrodynamic parameters obtained for different proteins has been made.
摘要
研究了蛋白质浓度和温度对羊血清白蛋白（OSA）溶液粘度的影响。分别使用穆尼方程和修正的阿累尼乌斯公式来描述溶液的粘度-浓度和粘度-温度依赖性。计算了水合OSA的有效比容、粘性流动的活化能和熵。确定了水合OSA的轴比和主半轴尺寸。在低浓度极限下，给出了特性粘度和哈金斯系数的温度依赖性。对不同蛋白质获得的一些流体动力学参数进行了比较。
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