碱性成纤维细胞生长因子对 MCF-7 细胞周期中 CD44(+)/CD24(-)的影响：促进 G0/G1→G2/S 期转变。
Effect of bFGF on the MCF-7 Cell Cycle with CD44(+)/CD24(-): Promoting the G0/G1→G2/S Transition.
机构信息
The First Affilated Hospital of Binzhou Medical University, Binzhou, China.
出版信息
J Breast Cancer. 2012 Dec;15(4):388-92. doi: 10.4048/jbc.2012.15.4.388. Epub 2012 Dec 31.
PURPOSE
Few cells with stem cell characteristics possess capabilities of self-renewal and differentiation, which leads to high tumorigenesis and resistance to standard chemotherapeutic agents. These cells are mostly quiescent, and arrest occurs at the mitotic G0/G1 phase in mitosis. We explored the effects of basic fibroblast growth factor (bFGF) on the MCF-7 cell cycle with CD44(+)/CD24(-).
METHODS
Cancer-initiating cells were propagated as mammospheres. The CD44(+)/CD24(-) subpopulation was sorted by a fluorescence activating cell sorter-Vantage flow cytometer. A cell cycle analysis was performed with different bFGF concentrations.
RESULTS
Differences in the CD44(+)/CD24(-) cell proliferation under different bFGF concentrations were observed (p=0.001). When the bFGF concentration was increased, the proportion of CD44(+)/CD24(-) at G0/G1 decreased (p=0.023).
CONCLUSION
We conclude that bFGF may sustain CD44(+)/CD24(-) cell proliferation and could promote cell progression through the G0/G1→G2/S phase transition.
目的
具有干细胞特征的细胞很少具有自我更新和分化的能力，这导致了高肿瘤发生和对标准化疗药物的耐药性。这些细胞大多处于静止状态，在有丝分裂 G0/G1 期停滞。我们探讨了碱性成纤维细胞生长因子 (bFGF) 对 MCF-7 细胞周期中 CD44(+)/CD24(-)的影响。
方法
以乳腺球体的形式增殖起始癌细胞。通过荧光激活细胞分选器-Vantage 流式细胞仪对 CD44(+)/CD24(-)亚群进行分选。用不同浓度的 bFGF 进行细胞周期分析。
结果
观察到不同 bFGF 浓度下 CD44(+)/CD24(-)细胞增殖的差异（p=0.001）。随着 bFGF 浓度的增加，G0/G1 期的 CD44(+)/CD24(-) 比例降低（p=0.023）。
结论
我们得出结论，bFGF 可能维持 CD44(+)/CD24(-)细胞的增殖，并通过 G0/G1→G2/S 期转变促进细胞进展。
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