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碱性成纤维细胞生长因子对 MCF-7 细胞周期中 CD44(+)/CD24(-)的影响：促进 G0/G1→G2/S 期转变。

Effect of bFGF on the MCF-7 Cell Cycle with CD44(+)/CD24(-): Promoting the G0/G1→G2/S Transition.

机构信息

The First Affilated Hospital of Binzhou Medical University, Binzhou, China.

出版信息

J Breast Cancer. 2012 Dec;15(4):388-92. doi: 10.4048/jbc.2012.15.4.388. Epub 2012 Dec 31.

DOI:10.4048/jbc.2012.15.4.388
PMID:23346166
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3542845/
Abstract

PURPOSE

Few cells with stem cell characteristics possess capabilities of self-renewal and differentiation, which leads to high tumorigenesis and resistance to standard chemotherapeutic agents. These cells are mostly quiescent, and arrest occurs at the mitotic G0/G1 phase in mitosis. We explored the effects of basic fibroblast growth factor (bFGF) on the MCF-7 cell cycle with CD44(+)/CD24(-).

METHODS

Cancer-initiating cells were propagated as mammospheres. The CD44(+)/CD24(-) subpopulation was sorted by a fluorescence activating cell sorter-Vantage flow cytometer. A cell cycle analysis was performed with different bFGF concentrations.

RESULTS

Differences in the CD44(+)/CD24(-) cell proliferation under different bFGF concentrations were observed (p=0.001). When the bFGF concentration was increased, the proportion of CD44(+)/CD24(-) at G0/G1 decreased (p=0.023).

CONCLUSION

We conclude that bFGF may sustain CD44(+)/CD24(-) cell proliferation and could promote cell progression through the G0/G1→G2/S phase transition.

摘要

目的

具有干细胞特征的细胞很少具有自我更新和分化的能力，这导致了高肿瘤发生和对标准化疗药物的耐药性。这些细胞大多处于静止状态，在有丝分裂 G0/G1 期停滞。我们探讨了碱性成纤维细胞生长因子 (bFGF) 对 MCF-7 细胞周期中 CD44(+)/CD24(-)的影响。

方法

以乳腺球体的形式增殖起始癌细胞。通过荧光激活细胞分选器-Vantage 流式细胞仪对 CD44(+)/CD24(-)亚群进行分选。用不同浓度的 bFGF 进行细胞周期分析。

结果

观察到不同 bFGF 浓度下 CD44(+)/CD24(-)细胞增殖的差异（p=0.001）。随着 bFGF 浓度的增加，G0/G1 期的 CD44(+)/CD24(-) 比例降低（p=0.023）。

结论

我们得出结论，bFGF 可能维持 CD44(+)/CD24(-)细胞的增殖，并通过 G0/G1→G2/S 期转变促进细胞进展。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3109/3542845/25baab8ffde0/jbc-15-388-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3109/3542845/bd94af26d943/jbc-15-388-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3109/3542845/f2668e419f2b/jbc-15-388-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3109/3542845/b529a504a3c0/jbc-15-388-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3109/3542845/25baab8ffde0/jbc-15-388-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3109/3542845/bd94af26d943/jbc-15-388-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3109/3542845/f2668e419f2b/jbc-15-388-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3109/3542845/b529a504a3c0/jbc-15-388-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3109/3542845/25baab8ffde0/jbc-15-388-g004.jpg

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Mammosphere culture of metastatic breast cancer cells enriches for tumorigenic breast cancer cells.转移性乳腺癌细胞的乳腺球培养可富集致瘤性乳腺癌细胞。
The effect of amniotic membrane extract on umbilical cord blood mesenchymal stem cell expansion: is there any need to save the amniotic membrane besides the umbilical cord blood?
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