Minton A P, Sokolovsky M
Laboratory of Biochemical Pharmacology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Biochemistry. 1990 Feb 13;29(6):1586-93. doi: 10.1021/bi00458a033.
The binding of the agonist carbamylcholine to muscarinic receptors in rat heart myocytes from young and aged cultures and in rat atrial membranes has been measured in the absence and presence of GppNHp, pertussis toxin, and/or batrachotoxin. The effect of each of the added substances upon agonist binding was accounted for by a model according to which the receptor may form an equilibrium complex with agonist and either of two distinct effector substances, one of which is postulated to increase the affinity of receptor for agonist and the other of which is postulated to decrease the affinity of receptor for agonist.
在不存在和存在GppNHp、百日咳毒素和/或蛙皮毒素的情况下，已测定了激动剂氨甲酰胆碱与来自年轻和老龄培养物的大鼠心肌细胞以及大鼠心房膜中的毒蕈碱受体的结合情况。每种添加物质对激动剂结合的影响由一个模型来解释，根据该模型，受体可能与激动剂以及两种不同效应物质中的任何一种形成平衡复合物，其中一种效应物质被假定会增加受体对激动剂的亲和力，另一种效应物质被假定会降低受体对激动剂的亲和力。
