Department of Oral Microbiology and Immunology, College of Dentistry, Dankook University, Cheonan, Korea.
Mol Oral Microbiol. 2013 Jun;28(3):230-8. doi: 10.1111/omi.12020. Epub 2013 Jan 24.
Porphyromonas gingivalis is associated with chronic periodontitis and forms multi-species biofilms. They can communicate within species as well as with other species found in the subgingiva, which may induce changes in the growth ratio and virulence of periodontopathogens. The lipopolysaccharide (LPS) of P. gingivalis shows different virulence by growth condition. The purpose of this study was to investigate the characteristics of P. gingivalis LPS when co-cultured with Fusobacterium nucleatum. After culture of P. gingivalis in the presence or absence of F. nucleatum, P. gingivalis LPS was extracted. THP-1 cells were treated with the LPS and induction of cytokine expression was investigated using real-time reverse transcription polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA). For the analysis of P. gingivalis LPS, LPS biosynthesis-related genes such as lpxA and lpxD were evaluated with real-time RT-PCR. Finally, molecular mass of lipid A was measured by mass spectrometry after hydrolysis of the LPS. Co-cultured P. gingivalis LPS exhibited higher induction of expression of interleukin 1β, 6, and 8 than single-cultured P. gingivalis LPS. These symptoms may be caused by an increase in m/z 1689 lipid A through the upregulation of lpxA and lpxD expression by communication between P. gingivalis and F. nucleatum.
牙龈卟啉单胞菌与慢性牙周炎有关，并形成多物种生物膜。它们可以在物种内以及在下颌龈下发现的其他物种之间进行通信，这可能会诱导牙周病原菌生长比例和毒力的变化。牙龈卟啉单胞菌的脂多糖（LPS）通过生长条件表现出不同的毒力。本研究旨在研究牙龈卟啉单胞菌与核梭杆菌共培养时 LPS 的特征。在存在或不存在核梭杆菌的情况下培养牙龈卟啉单胞菌后，提取牙龈卟啉单胞菌 LPS。用 LPS 处理 THP-1 细胞，并通过实时逆转录聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）研究细胞因子表达的诱导。为了分析牙龈卟啉单胞菌 LPS，使用实时 RT-PCR 评估 LPS 生物合成相关基因，如 lpxA 和 lpxD。最后，通过 LPS 水解后测量质荷比（m/z）1689 的脂质 A 的质谱法测量。与单一培养的牙龈卟啉单胞菌 LPS 相比，共培养的牙龈卟啉单胞菌 LPS 诱导表达白细胞介素 1β、6 和 8 的能力更高。这些症状可能是由于通过牙龈卟啉单胞菌和核梭杆菌之间的通信上调 lpxA 和 lpxD 表达，导致 m/z 1689 脂质 A 增加所致。
