L. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wrocław, Poland.
Carbohydr Res. 2013 Mar 7;368:84-8. doi: 10.1016/j.carres.2012.12.016. Epub 2012 Dec 27.
Mild acid degradation of the lipopolysaccharide of Edwardsiella tarda PCM 1150 afforded an O-polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar and methylation analyses along with 1D and 2D 1H and 13C NMR spectroscopies, including experiments performed in a 9:1 H2O/D2O mixture to detect NH protons and their correlations with CH protons. The O-polysaccharide was found to contain an amide of d-glucuronic acid with l-alanine (d-GlcA6Ala) and the following structure of the branched hexasaccharide repeating unit was established: -->4)-β-D-GlepA6Ala-(1-->4)-α-L-Fucp-(1-->4)-α-D-Glcp-(1-->4)-α-D-Quip-(1-->3)-β-D-GlcpNAc-(1-->3<--1α-D-GalpNAc.
温和酸降解迟缓爱德华氏菌 PCM 1150 的脂多糖得到一个 O-多糖，经 GPC 在 Sephadex G-50 上分离，并通过糖和甲基化分析以及 1D 和 2D 1H 和 13C NMR 光谱研究，包括在 9:1 H2O/D2O 混合物中进行的实验，以检测 NH 质子及其与 CH 质子的相关性。O-多糖被发现含有 d-葡萄糖醛酸与 l-丙氨酸（d-GlcA6Ala）的酰胺，并且建立了支化六糖重复单元的以下结构：-->4)-β-D-GlepA6Ala-(1-->4)-α-L-Fucp-(1-->4)-α-D-Glcp-(1-->4)-α-D-Quip-(1-->3)-β-D-GlcpNAc-(1-->3<--1α-D-GalpNAc。
