Laboratory of Clinical Pharmaceutics & Therapeutics, Division of Pharmasciences, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060-0812, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Feb 15;917-918:18-23. doi: 10.1016/j.jchromb.2012.11.024. Epub 2012 Dec 3.
Carboplatin is a platinum agent that is used for treatment of non-small-cell lung cancer and ovarian cancer. A sensitive and selective analytical method for the quantification of carboplatin in human plasma ultrafiltrates using liquid chromatography-tandem mass spectrometry was developed. Human plasma ultrafiltrates were precipitated by acetonitrile containing carboplatin-d4 as an internal standard and were further diluted with acetonitrile. Chromatographic separation was performed on a Accucore HILIC (50mm×2.1mm i.d., 2.6μm) column using mobile phase (acetonitrile-water-acetic acid=90:10:0.1, v/v/v) at the flow rate of 0.2mL/min. Detection was performed on electrospray ionization triple quadrupole tandem mass spectrometer using low-energy collision induced dissociation (CID-MS/MS) analysis operating in the selected reaction monitoring (SRM) scan mode. The lower limit of quantification for carboplatin was 0.025μg/mL. This method covered a linearity range of 0.025-50μg/mL. The intra-day precision and inter-day precision (R.S.D.) ranged from 1.5 to 4.3%, and the accuracy (R.E.) was within ±2.9%. The present method was applied to a clinical pharmacokinetic study of carboplatin in a cancer patient.
卡铂是一种铂类药物，用于治疗非小细胞肺癌和卵巢癌。建立了一种灵敏、专属性的液相色谱-串联质谱法，用于人血浆超滤液中卡铂的定量分析。用人血浆超滤液沉淀含卡铂-d4（内标）的乙腈，再用乙腈稀释。采用 Accucore HILIC（50mm×2.1mm i.d.，2.6μm）柱，以乙腈-水-冰醋酸（90:10:0.1，v/v/v）为流动相，流速为 0.2mL/min 进行色谱分离。在电喷雾电离三重四极杆串联质谱仪上，采用低能量碰撞诱导解离（CID-MS/MS）分析，在选择反应监测（SRM）扫描模式下进行检测。卡铂的定量下限为 0.025μg/mL。该方法的线性范围为 0.025-50μg/mL。日内精密度和日间精密度（RSD）为 1.5%-4.3%，准确度（RE）为±2.9%。该方法应用于癌症患者卡铂的临床药代动力学研究。
