University of Hawaii, Department of Cell and Molecular Biology, John A. Burns School of Medicine, 651 Ilalo St., BSB222, Honolulu, HI 96813, USA.
Methods. 2013 Mar;59(3):278-86. doi: 10.1016/j.ymeth.2013.01.004. Epub 2013 Jan 22.
Protein interactions are critical for biological specificity and techniques able to characterize these interactions are of fundamental importance in biochemistry and cell biology. Fluorescence methodologies have been extremely useful for studying many biological systems including protein-ligand and protein-protein interactions. In this review we focus on the application of time-resolved fluorescence approaches to macromolecular systems. We also include a detailed discussion of a relatively new time-resolved technique, the phasor method, for studying protein interactions both in vitro and in live cells.
蛋白质相互作用对于生物特异性至关重要，能够描述这些相互作用的技术在生物化学和细胞生物学中具有重要意义。荧光方法在研究许多生物系统方面非常有用，包括蛋白质-配体和蛋白质-蛋白质相互作用。在这篇综述中，我们专注于时间分辨荧光方法在大分子系统中的应用。我们还详细讨论了一种相对较新的时间分辨技术——相面法，用于研究体外和活细胞中的蛋白质相互作用。
