National Food Institute, Technical University of Denmark, Department of Toxicology and Risk Assessment, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark.
Food Chem Toxicol. 2013 May;55:265-71. doi: 10.1016/j.fct.2012.12.060. Epub 2013 Jan 21.
Phytoestrogens (PEs) are naturally occurring plant components, with the ability to induce biological responses in vertebrates by mimicking or modulating the action of endogenous hormones. Single isoflavones have been shown to affect adipocyte differentiation, but knowledge on the effect of dietary relevant mixtures of PEs, including for instance lignans, is lacking. In the current study dietary relevant mixtures of isoflavones and their metabolites, lignans and their metabolites, coumestrol, and a mixture containing all of them, were examined for effects on adipogenesis in 3T3-L1 adipocytes, as well as tested for their PPARγ activating abilities. The results showed that mixtures of isoflavonoid parent compounds and metabolites, respectively, a mixture of lignan metabolites, as well as coumestrol concentration-dependently inhibited adipocyte differentiation. Furthermore, a mixture of isoflavonoid parent compounds, and a mixture of isoflavonoid metabolites were found to have PPARγ activating abilities. These results suggest that PEs can affect pathways known to play a role in obesity development, and indicate that the inhibitory effect on adipocyte differentiation does not appear to be strictly associated with PPARγ activation/inhibition. The current study support the hypothesis that compounds with endocrine activity can affect pathways playing a role in the development obesity and obesity related diseases.
植物雌激素（PEs）是天然存在的植物成分，通过模拟或调节内源性激素的作用，具有诱导脊椎动物产生生物学反应的能力。已经表明，单一的异黄酮能够影响脂肪细胞的分化，但是对于饮食中相关的 PEs 混合物（例如木脂素）的影响，我们还知之甚少。在当前的研究中，我们研究了饮食中相关的异黄酮及其代谢物、木脂素及其代谢物、香豆雌酚以及包含所有这些物质的混合物对 3T3-L1 脂肪细胞脂肪生成的影响，同时测试了它们对 PPARγ 的激活能力。结果表明，异黄酮母体化合物和代谢物的混合物、木脂素代谢物的混合物以及香豆雌酚浓度依赖性地抑制脂肪细胞分化。此外，还发现异黄酮母体化合物的混合物和异黄酮代谢物的混合物具有 PPARγ 激活能力。这些结果表明，PEs 可以影响已知在肥胖发展中起作用的途径，并表明对脂肪细胞分化的抑制作用似乎与 PPARγ 的激活/抑制无关。本研究支持这样一种假设，即具有内分泌活性的化合物可以影响在肥胖和肥胖相关疾病发展中起作用的途径。
