亚马孙地区美洲印第安人的 Y 连锁微卫星在巴西非洲裔人群的祖先估计中的应用。

Y-linked microsatellites in Amazonian Amerindians applied to ancestry estimates in Brazilian Afro-derived populations.

机构信息

Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.

出版信息

Am J Hum Biol. 2013 May-Jun;25(3):313-7. doi: 10.1002/ajhb.22361. Epub 2013 Jan 24.

DOI:10.1002/ajhb.22361

PMID:23348861

Abstract

OBJECTIVES

Proper ancestral populations are required to determine accurate ancestry estimates for Afro-derived Brazilian populations. Herein, we have genotyped Y-STRs in Amazonian Amerindians to determine the ancestral contribution in quilombo remnant communities.

METHODS

The frequencies for five Y-chromosome linked microsatellites (DYS19, DYS390, DYS391, DYS392, and DYS393) were characterized in four Amerindian tribes from Brazilian Amazon (Tikúna, Baníwa, Kashinawa, and Kanamarí), and in four quilombo remnants (Mimbó, Sítio Velho, Gaucinha, and São Gonçalo) and two urban populations (Teresina and Jequié) from Northeastern Brazil. We then estimated the male genetic ancestry in each admixed population. Moreover, we performed analysis of molecular variance (AMOVA), FST , haplotype diversity, and principal component analysis.

RESULTS

Lower haplotype diversity (h) values were observed for Tikúna compared with other tribes. Quilombo remnants exhibited higher h levels ranging from 0.893 ± 0.027 in Sítio Velho to 0.963 ± 0.033 in São Gonçalo. African ancestry estimates ranged from 0.529 ± 0.027 in Mimbó to 0.602 ± 0.086 in Sítio Velho. Conversely, European contribution was 0.795 ± 0.045 in Teresina and 0.826 ± 0.040 in Jequié.

CONCLUSIONS

FST and principal component analysis indicate homogeneity in the male genetic constitution among the quilombo remnants analyzed. Data on Amerindians allowed accurate ancestry estimates, which indicated a higher African contribution, followed by a considerable European contribution for these quilombo remnants.

摘要

目的

为了准确估计非裔巴西人群的祖先，需要使用合适的祖先群体。在此，我们对亚马孙地区的美洲原住民进行了 Y 染色体 STR 基因分型，以确定 quilombo 残余社区的祖先贡献。

方法

本研究对来自巴西亚马孙地区的四个美洲原住民部落（Tikúna、Baníwa、Kashinawa 和 Kanamarí）以及四个 quilombo 残余社区（Mimbó、Sítio Velho、Gaucinha 和 São Gonçalo）和两个来自巴西东北部的城市人口（特雷西纳和若基埃）的五个 Y 染色体连锁微卫星（DYS19、DYS390、DYS391、DYS392 和 DYS393）的频率进行了特征描述。然后，我们估计了每个混合人群中的男性遗传祖先。此外，我们进行了分子方差分析（AMOVA）、FST、单倍型多样性和主成分分析。

结果

与其他部落相比，Tikúna 的单倍型多样性（h）值较低。quilombo 残余社区表现出较高的 h 值，范围从 Sítio Velho 的 0.893±0.027 到 São Gonçalo 的 0.963±0.033。非洲祖先的估计值范围从 Mimbó 的 0.529±0.027 到 Sítio Velho 的 0.602±0.086。相反，欧洲的贡献在特雷西纳为 0.795±0.045，在若基埃为 0.826±0.040。