Boyd J A, Kaufman D G
Department of Pathology, School of Medicine, University of North Carolina, Chapel Hill 27599.
Cancer Res. 1990 Jun 1;50(11):3394-9.
We examined the effects of transforming growth factor beta 1 (TGF-beta 1) on various aspects of the cell biology of human endometrial carcinoma (HEC) cell lines in vitro, as well as the expression of TGF-beta 1 mRNA by these cell lines. Cell lines from eight HEC tumors, representing a variety of histological subtypes, were studied in order to test the generality of conclusions regarding the effects of TGF-beta 1 on this particular tumor cell type. The growth of five HEC cell lines was inhibited by TGF-beta 1 (10 ng/ml), while growth of three cell lines was not inhibited. The effects on growth correlated with morphological alterations induced by TGF-beta 1; the cell lines with inhibited growth displayed a larger, flatter, more contact-inhibited phenotype, while the cell lines whose growth ws not inhibited showed few discernible morphological alterations in response to TGF-beta 1. Northern analysis of TGF-beta 1 mRNA levels revealed that the three HEC cell lines unresponsive to TGF-beta 1 treatment expressed relatively large amounts of TGF-beta 1. Correspondingly, the five HEC cell lines which responded to TGF-beta 1 with growth and morphological changes expressed much lower levels of TGF-beta 1 mRNA. These results suggest that the sensitivity of human HEC cell lines to TGF-beta 1 is variable and that this sensitivity is inversely correlated with the level of expression of TGF-beta 1.
我们研究了转化生长因子β1（TGF-β1）对人子宫内膜癌（HEC）细胞系体外细胞生物学各个方面的影响，以及这些细胞系中TGF-β1 mRNA的表达。研究了来自8例HEC肿瘤的细胞系，它们代表了多种组织学亚型，以检验关于TGF-β1对这种特定肿瘤细胞类型影响的结论的普遍性。5种HEC细胞系的生长受到TGF-β1（10 ng/ml）的抑制，而3种细胞系的生长未受抑制。对生长的影响与TGF-β1诱导的形态学改变相关；生长受抑制的细胞系表现出更大、更扁平、接触抑制更明显的表型，而生长未受抑制的细胞系对TGF-β1几乎没有明显的形态学改变。对TGF-β1 mRNA水平的Northern分析显示，3种对TGF-β1处理无反应的HEC细胞系表达相对大量的TGF-β1。相应地，5种对TGF-β1有生长和形态学变化反应的HEC细胞系表达的TGF-β1 mRNA水平要低得多。这些结果表明，人HEC细胞系对TGF-β1的敏感性是可变的，且这种敏感性与TGF-β1的表达水平呈负相关。
