Endocrine Research Unit, Division of Endocrinology, Diabetes, Metabolism and Nutrition, Mayo Clinic, Rochester, Minnesota, USA.
Diabetes. 2013 Jun;62(6):1897-903. doi: 10.2337/db12-1407. Epub 2013 Jan 24.
Spillover of lipoprotein lipase-generated fatty acids from chylomicrons into the plasma free fatty acid (FFA) pool is an important source of FFA and reflects inefficiency in dietary fat storage. We measured spillover in 13 people with type 2 diabetes using infusions of a [(3)H]triolein-labeled lipid emulsion and [U-(13)C]oleate during continuous feeding, before and after weight loss. Body fat was measured with dual energy X-ray absorptiometry and computed tomography. Participants lost ∼14% of body weight. There was an ∼38% decrease in meal-suppressed FFA concentration (P < 0.0001) and an ∼23% decrease in oleate flux (P = 0.007). Fractional spillover did not change (P = NS). At baseline, there was a strong negative correlation between spillover and leg fat (r = -0.79, P = 0.001) and a positive correlation with the trunk-to-leg fat ratio (R = 0.56, P = 0.047). These correlations disappeared after weight loss. Baseline leg fat (R = -0.61, P = 0.027) but not trunk fat (R = -0.27, P = 0.38) negatively predicted decreases in spillover with weight loss. These results indicate that spillover, a measure of inefficiency in dietary fat storage, is inversely associated with lower body fat in type 2 diabetes.
乳糜微粒衍生的脂蛋白脂肪酶生成的脂肪酸向血浆游离脂肪酸 (FFA) 池的溢出是 FFA 的重要来源，反映了膳食脂肪储存效率低下。我们使用 [(3)H]三油酸甘油酯标记的脂质乳剂和 [U-(13)C]油酸输注，在持续喂养前后，在 13 名 2 型糖尿病患者中测量了这种溢出。使用双能 X 射线吸收法和计算机断层扫描测量体脂。参与者体重减轻了约 14%。膳食抑制的 FFA 浓度下降了约 38%（P < 0.0001），油酸通量下降了约 23%（P = 0.007）。分数溢出没有变化（P = NS）。在基线时，溢出与腿部脂肪呈强烈负相关（r = -0.79，P = 0.001），与躯干与腿部脂肪比例呈正相关（r = 0.56，P = 0.047）。减肥后这些相关性消失。基线腿部脂肪（r = -0.61，P = 0.027）而不是躯干脂肪（r = -0.27，P = 0.38）与减肥时溢出减少呈负相关。这些结果表明，溢出是膳食脂肪储存效率低下的一种衡量标准，与 2 型糖尿病患者较低的体脂呈负相关。
