Unité Environnement-Santé, Institut Pasteur de la Guadeloupe, Les Abymes, Guadeloupe, France.
PLoS One. 2013;8(1):e54414. doi: 10.1371/journal.pone.0054414. Epub 2013 Jan 18.
In 2008 a fatal case of primary amoebic meningoencephalitis, due to the amoeboflagellate Naegleria fowleri, occurred in Guadeloupe, French West Indies, after a child swam in a bath fed with geothermal water. In order to improve the knowledge on free-living amoebae in this tropical part of France, we investigated on a monthly basis, the presence of Naegleria spp. in the recreational baths, and stream waters which feed them. A total of 73 water samples, 48 sediments and 54 swabs samples were collected from 6 sampling points between June 2011 and July 2012. The water samples were filtered and the filters transferred to non-nutrient agar plates seeded with a heat-killed suspension of Escherichia coli while sediment and swab samples were placed directly on these plates. The plates were incubated at 44°C for the selective isolation of thermophilic Naegleria. To identify the Naegleria isolates the internal transcribed spacers, including the 5.8S rDNA, were amplified by polymerase chain reaction and the sequence of the PCR products was determined. Thermophilic amoebae were present at nearly all collection sites. The pathogenic N. fowleri was the most frequently encountered thermophilic species followed by N. lovaniensis. The concentration of N. fowleri was rather low in most water samples, ranging from 0 to 22 per liter. Sequencing revealed that all N. fowleri isolates belonged to a common Euro-American genotype, the same as detected in the human case in Guadeloupe. These investigations need to be continued in order to counsel the health authorities about prevention measures, because these recreational thermal baths are used daily by local people and tourists.
2008 年，在法属西印度群岛瓜德罗普，一名儿童在一处由地热水供应的浴场游泳后，感染福氏耐格里阿米巴原虫，患上原发性阿米巴脑膜脑炎，最终死亡。为了增进对法国这一热带地区自由生活阿米巴的认识，我们每月对这些浴场和为其供水的溪流中的耐格里属进行调查。2011 年 6 月至 2012 年 7 月，我们从 6 个采样点共采集了 73 份水样、48 份沉积物样本和 54 份拭子样本。水样经过滤后，将滤器转移到非营养琼脂平板上，平板上接种了经热灭活的大肠杆菌悬液；沉积物和拭子样本则直接放在这些平板上。平板在 44°C 下孵育，以选择性分离嗜热耐格里属。为了鉴定耐格里属分离株，我们通过聚合酶链反应扩增了包括 5.8S rDNA 在内的内部转录间隔区，并确定了 PCR 产物的序列。几乎所有采集点都存在嗜热阿米巴。致病性福氏耐格里阿米巴是最常遇到的嗜热种，其次是洛瓦尼氏耐格里阿米巴。大多数水样中福氏耐格里阿米巴的浓度都相当低，范围从每升 0 到 22 个。测序显示，所有福氏耐格里阿米巴分离株都属于一种常见的欧美基因型，与在瓜德罗普的人类病例中检测到的相同。这些研究需要继续进行，以便向卫生当局提供预防措施方面的建议，因为这些休闲热浴场被当地人和游客每天使用。
