Smith C A, Xu Y Z, Swann P F
Department of Biochemistry, University College and Middlesex School of Medicine, London, UK.
Carcinogenesis. 1990 May;11(5):811-6. doi: 10.1093/carcin/11.5.811.
Practical methods for the routine solid-phase synthesis of oligodeoxynucleotides containing O6-alkylguanine are described. It is shown that if the 2-amino group of the alkylated base is protected with a phenylacetyl group, and a mixture of nitrobenzaldoximate ions and ammonia used to remove the protecting groups from the oligomer at the end of the synthesis, there is negligible formation of 2,6-diaminopurine and that after chromatography pure oligomers are obtained.
描述了用于常规固相合成含O6-烷基鸟嘌呤的寡脱氧核苷酸的实用方法。结果表明，如果烷基化碱基的2-氨基用苯乙酰基保护，并且在合成结束时使用硝基苯甲醛肟离子和氨的混合物从寡聚物上去除保护基，则2,6-二氨基嘌呤的形成可忽略不计，并且在色谱分离后可获得纯的寡聚物。
