Fralix T A, Heineman F W, Balaban R S
Laboratory of Cardiac Energetics, NIH, Bethesda, MD 20892.
FEBS Lett. 1990 Mar 26;262(2):287-92. doi: 10.1016/0014-5793(90)80212-2.
The effects of tissue optical absorbance on intracellular NAD(P)H and Indo-1 fluorescence emission have been evaluated in the perfused rabbit heart. These results demonstrate that the tissue optical absorbance significantly modifies the emission characteristics of these fluorophores. This tissue 'inner filter' effect, observed with both probes, changed as a function of tissue oxygenation and redox state in a wavelength-dependent manner. Pathlength calculations from these results indicate that this inner filter effect could occur with a mean pathlength of 310 microns due to the extremely high extinction coefficient of heart tissue. It is concluded that tissue optical absorbance significantly affects the fluorescent emission characteristics of both intrinsic and extrinsic probes in the intact heart, under a variety of conditions. Several potential methods of correcting for these tissue inner filter effects are discussed.
在灌注兔心脏中评估了组织光学吸光度对细胞内烟酰胺腺嘌呤二核苷酸（磷酸）（NAD(P)H）和吲哚菁绿-1（Indo-1）荧光发射的影响。这些结果表明，组织光学吸光度显著改变了这些荧光团的发射特性。使用这两种探针均观察到的这种组织“内滤光片”效应，随组织氧合和氧化还原状态以波长依赖的方式变化。根据这些结果进行的光程计算表明，由于心脏组织的极高消光系数，这种内滤光片效应可能在平均光程为310微米时发生。得出的结论是，在各种条件下，组织光学吸光度会显著影响完整心脏中内源性和外源性探针的荧光发射特性。讨论了几种校正这些组织内滤光片效应的潜在方法。
