Krüttgen A, Rose-John S, Möller C, Wroblowski B, Wollmer A, Müllberg J, Hirano T, Kishimoto T, Heinrich P C
Institut für Biochemie der RWTH Aachen, FRG.
FEBS Lett. 1990 Mar 26;262(2):323-6. doi: 10.1016/0014-5793(90)80219-9.
C-terminally deleted analogs of human interleukin-6 (IL-6) have been constructed at the cDNA level, and after cell-free transcription and translation their biological activity was analyzed. Removal of only 4 amino acids resulted in complete loss of biological activity as determined by the B9 cell proliferation assay. Secondary structure prediction of human IL-6 resulted in 58% helix, 14% beta-structure, and 28% turn and coil (average of 3 independent methods). The circular dichroism of recombinant human IL-6 was measured in the near and far UV. Evaluation of the latter in terms of secondary structures gave 67% helix, 15% beta-structure, and 18% turn and coil.
已在cDNA水平构建了人白细胞介素-6（IL-6）的C末端缺失类似物，并在无细胞转录和翻译后分析了它们的生物学活性。通过B9细胞增殖试验确定，仅去除4个氨基酸就导致生物活性完全丧失。人IL-6的二级结构预测结果为58%的螺旋结构、14%的β结构以及28%的转角和卷曲结构（3种独立方法的平均值）。在近紫外和远紫外区域测量了重组人IL-6的圆二色性。根据二级结构对后者进行评估，结果为67%的螺旋结构、15%的β结构以及18%的转角和卷曲结构。
