在双胎妊娠中，OPN 在胎盘组织中的表达与胎儿生长不一致。

Placental expression of osteopontin(OPN) in monochorionic twins with discordant growth.

机构信息

Fatal Medicine Center, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China.

出版信息

Placenta. 2013 Mar;34(3):288-90. doi: 10.1016/j.placenta.2012.12.014. Epub 2013 Jan 22.

DOI:10.1016/j.placenta.2012.12.014

Abstract

Shallow invasion could result in pathological processes of placenta leading to fetal growth restriction (FGR) in singletons and twins. Osteopontin (OPN) plays an important role in trophoblast invasion. So our study aims to investigate the expression level of OPN in placenta of discordant monochorionic (MC) twins. OPN expression was compared in the placental samples of 10 discordant MC twins and 12 concordant MC twins. OPN levels were evaluated using quantitative Real-time PCR and western blot. Our results showed that OPN expression at mRNA and protein level was significantly decreased in placenta (p < 0.05) of small fetuses in discordant MC twins. The expression level of OPN transcript strongly correlated with the territory of placenta.

摘要

浅着床可导致胎盘发生病理性改变，从而导致单胎和双胎的胎儿生长受限（FGR）。骨桥蛋白（OPN）在滋养细胞侵袭中发挥重要作用。因此，我们的研究旨在探讨不均一性单绒毛膜（MC）双胞胎胎盘组织中 OPN 的表达水平。比较了 10 例不均一性 MC 双胞胎和 12 例一致性 MC 双胞胎胎盘组织中的 OPN 表达。采用定量实时 PCR 和 Western blot 法评估 OPN 水平。结果显示，在不一致性 MC 双胞胎中，小胎儿胎盘组织中 OPN 的 mRNA 和蛋白水平表达显著降低（p < 0.05）。OPN 转录本的表达水平与胎盘的面积强烈相关。