氨基糖对猪肾海藻糖酶的时间依赖性抑制作用。

Time-dependent inhibition of porcine kidney trehalase by aminosugars.

作者信息

Salleh H M, Honek J F

机构信息

Guelph-Waterloo Centre for Graduate Work in Chemistry, University of Waterloo, Ont. Canada.

出版信息

FEBS Lett. 1990 Mar 26;262(2):359-62. doi: 10.1016/0014-5793(90)80229-c.

DOI:10.1016/0014-5793(90)80229-c

PMID:2335220

Abstract

The inhibitory effects of various nitrogen-containing sugars on porcine kidney trehalase were studied. Validamycin A, validoxylamine A and MDL 25,637 were found to be potent, time-dependent inhibitors of the enzyme in vitro. The validoxylamine A-inhibited enzyme showed slow but reversible reactivation over time (t1/2 = 1.2 h). To our knowledge, this is the first report of time-dependent inhibition exhibited by either these particular aminosugars or a trehalase.

摘要

研究了各种含氮糖类对猪肾海藻糖酶的抑制作用。发现井冈霉素A、井冈霉胺A和MDL 25,637在体外是该酶的强效、时间依赖性抑制剂。经井冈霉胺A抑制的酶随时间显示出缓慢但可逆的复活（半衰期 = 1.2小时）。据我们所知，这是关于这些特定氨基糖或海藻糖酶表现出时间依赖性抑制的首次报道。