Center for Evolutionary Medicine and Informatics in the Biodesign Institute, and Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85287-5301, USA.
Bioorg Med Chem Lett. 2013 Mar 1;23(5):1447-9. doi: 10.1016/j.bmcl.2012.12.080. Epub 2013 Jan 5.
Threose nucleic acid (TNA) is an artificial genetic polymer in which the natural ribose sugar found in RNA has been replaced with an unnatural threose sugar. TNA can be synthesized enzymatically using Therminator DNA polymerase to copy DNA templates into TNA. Here, we expand the substrate repertoire of Therminator DNA polymerase to include threofuranosyl adenine 3'-triphsophate (tATP). We chemically synthesized tATP by two different methods from the 2'-O-acetyl derivative. Enzyme-mediated polymerization reveals that tATP functions as an efficient substrate for Therminator DNA polymerase, indicating that tATP can replace the diaminopurine analogue (tDTP) in TNA transcription reactions.
三碳核酸（TNA）是一种人工遗传聚合物，其中 RNA 中发现的天然核糖已被非天然的赤藓糖取代。TNA 可以使用 Therminator DNA 聚合酶通过酶促合成来复制 DNA 模板成 TNA。在这里，我们扩展了 Therminator DNA 聚合酶的底物谱，包括三呋喃糖基腺嘌呤 3'-三磷酸 (tATP)。我们通过两种不同的方法从 2'-O-乙酰衍生物化学合成 tATP。酶促聚合表明 tATP 是 Therminator DNA 聚合酶的有效底物，表明 tATP 可以替代 TNA 转录反应中的二氨基嘌呤类似物 (tDTP)。
