Hospital for Special Surgery, New York, New York, USA.
Arthroscopy. 2013 Mar;29(3):596-607. doi: 10.1016/j.arthro.2012.10.025. Epub 2013 Jan 24.
To perform a systematic review of the basic science literature on the use of platelet-rich plasma (PRP) in tendon models.
We searched the PubMed/Medline and Embase databases in June 2012 using the following parameters: ((tenocytes OR tendon OR tendinitis OR tendinosis OR tendinopathy) AND (platelet rich plasma OR PRP OR autologous conditioned plasma OR ACP)). The inclusion criteria for full-text review were in vivo and in vitro studies examining the effects of PRP on tendons and/or tenocytes. Clinical studies were excluded. Only studies published in peer-reviewed journals that compared PRP directly with a control were included. Data were extracted based on a predefined data sheet, which included information on PRP preparation, study methods, and results. Studies were analyzed for trends, comparing and contrasting the reported effects of PRP.
The search yielded 31 articles for inclusion in our review. Of the studies, 22 (71%) reported platelet concentrations in the PRP; 6 (19%) reported cytology. Eight in vivo studies found decreased tendon repair time, increased fiber organization, or both with PRP treatment. Eight in vitro studies reported that PRP treatment increased cell proliferation; 7 reported an increase in growth factor expression. Three in vivo studies found increased vascularity, and 4 found increased tensile strength with PRP treatment.
In the basic science studies evaluated, it appears that PRP confers several potential effects on tendon models compared with a control. However, the literature is inconsistent with regard to reporting the methods of preparation of PRP and in reporting platelet concentrations and cytology.
Establishing proof of concept for PRP may lead to further high-quality clinical studies in which the appropriate indications can be defined.
对使用富含血小板的血浆（PRP）治疗肌腱模型的基础科学文献进行系统评价。
我们于 2012 年 6 月在 PubMed/Medline 和 Embase 数据库中使用以下参数进行搜索：（（腱细胞或肌腱或肌腱炎或肌腱病或肌腱病）和（富含血小板的血浆或 PRP 或自体条件血浆或 ACP））。全文审查的纳入标准为研究 PRP 对肌腱和/或腱细胞影响的体内和体外研究。排除临床研究。仅纳入发表在同行评议期刊上的直接比较 PRP 与对照的研究。根据预定义的数据表提取数据，其中包括 PRP 制备、研究方法和结果的信息。分析研究趋势，比较和对比 PRP 的报告效果。
搜索结果为 31 篇文章纳入本综述。在这些研究中，22 项（71%）报告了 PRP 中的血小板浓度；6 项（19%）报告了细胞学。8 项体内研究发现 PRP 治疗可减少肌腱修复时间、增加纤维组织或两者兼有。8 项体外研究报告 PRP 治疗可增加细胞增殖；7 项报告生长因子表达增加。3 项体内研究发现 PRP 治疗后血管生成增加，4 项研究发现肌腱拉伸强度增加。
在评估的基础科学研究中，与对照组相比，PRP 似乎对肌腱模型具有多种潜在影响。然而，关于 PRP 制备方法的报告以及血小板浓度和细胞学的报告在文献中不一致。
为 PRP 建立概念验证可能会导致进一步的高质量临床研究，从而确定适当的适应证。
