Yin Qiaoxiang, Zhao Yusheng, Wang Heng, Pei Zhiyong
Department of Elderly Cardiology, General Hospital of Air Force, Beijing, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2013 Jan;33(1):34-9.
To assess a minipig model of acute myocardial infarction (AMI) established by percutaneous balloon occlusion using delayed-enhanced magnetic resonance imaging (DE-MRI).
A minipig model of AMI was established by placement of a 2.0 mm×15.0 mm percutaneous transluminal coronary angioplasty balloon in the middle left anterior descending artery (LAD) through a percutaneous femoral puncture in the right inguinal region. The left anterior descending coronary artery (LAD) was occluded for 90 min, followed by assessment of the infarct size and cardiac function with DE-MRI, and the results were confirmed by pathological examination.
DE-MRI showed a mean infarcts size of 10.2∓2.9 cm3 in the minipig models. Compared to the control group, the minipigs with AMI had significantly increased end-diastolic and end-systolic volumes (P<0.05) with a decreased stroke volume, ejection fraction and cardiac output (P<0.001). These DE-MRI values were matched with the microsphere values obtained from short-axis slices in pathological examination.
We have established a feasible approach for evaluating minipig models of AMI as a platform for assessing the therapeutic effect of stem cell transplantation for AMI.
使用延迟增强磁共振成像（DE-MRI）评估经皮球囊闭塞建立的小型猪急性心肌梗死（AMI）模型。
通过右腹股沟区经皮股动脉穿刺，将2.0 mm×15.0 mm的经皮腔内冠状动脉成形术球囊置于左前降支动脉（LAD）中段，建立小型猪AMI模型。将左冠状动脉前降支（LAD）闭塞90分钟，然后用DE-MRI评估梗死面积和心功能，并通过病理检查证实结果。
DE-MRI显示小型猪模型的平均梗死面积为10.2±2.9 cm3。与对照组相比，AMI小型猪的舒张末期和收缩末期容积显著增加（P<0.05），而每搏输出量、射血分数和心输出量降低（P<0.001）。这些DE-MRI值与病理检查中短轴切片获得的微球值相符。
我们已经建立了一种可行的方法来评估小型猪AMI模型，作为评估干细胞移植治疗AMI疗效的平台。
