通过酶联免疫吸附测定（ELISA）检测和鉴定干燥脾脏中的HLA抗原。

Detection and identification of HLA antigens in dried spleens by means of enzyme linked immunosorbent assay (ELISA).

作者信息

Bishara A, Brautbar C

机构信息

Department of Clinical Microbiology, Hadassah University Hospital, Jerusalem, Israel.

出版信息

Forensic Sci Int. 1990 Mar;45(1-2):97-105. doi: 10.1016/0379-0738(90)90226-o.

DOI:10.1016/0379-0738(90)90226-o

PMID:2335337

Abstract

HLA class-I and class-II antigens were detected in extracts of dry spleen tissue allowed to age from 1 to 17 months, using monomorphic anti HLA monoclonal antibodies and the ELISA technique. Using polymorphic HLA alloantisera samples of spleen tissue could be characterized and differentiated. The potential use of dried tissues and blood stains is discussed.

摘要

使用单克隆抗人白细胞抗原（HLA）单克隆抗体和酶联免疫吸附测定（ELISA）技术，对保存1至17个月的干燥脾脏组织提取物中的HLA I类和II类抗原进行检测。使用多态性HLA同种抗血清可对脾脏组织样本进行表征和区分。文中还讨论了干燥组织和血迹的潜在用途。