Lansdorp P M, Oosterhof F, Astaldi G C, Zeijlemaker W P
Tissue Antigens. 1982 Jan;19(1):11-9. doi: 10.1111/j.1399-0039.1982.tb01411.x.
The expression of HLA-A and -B antigens on peripheral blood lymphocytes and blood platelets was measured using monoclonal antibodies in a semi-quantitative ELISA technique. Reactively of monoclonal anti-HLA-A2 and anti-HLA-B7, with lymphocytes as well as platelets, was in agreement with the presence of these antigens as detected by conventional HLA typing of lymphocytes. When the actual amount of HLA antigens was measured, a gene-dose effect was seen: cells from HLA-B7-homozygous individuals bound more monoclonal anti-HLA-B7 antibodies compared to their HLA-B7-heterozygous siblings. At the same time, cells of different donors showed only very small differences in binding of monoclonal antibody against an HLA-"backbone" determinant. Relative to total HLA-A, -B and -C expression, the amounts of HLA-A2 on lymphocytes and platelets were similar. On the other hand, the expression of HLA-B7 on platelets was diminished compared to that on peripheral blood lymphocytes.
采用单克隆抗体，运用半定量酶联免疫吸附测定技术检测外周血淋巴细胞和血小板上HLA - A和 - B抗原的表达。单克隆抗HLA - A2和抗HLA - B7与淋巴细胞以及血小板的反应性，与通过淋巴细胞常规HLA分型检测到的这些抗原的存在情况一致。当测量HLA抗原的实际量时，观察到基因剂量效应：与HLA - B7杂合子的同胞相比，来自HLA - B7纯合子个体的细胞结合更多的单克隆抗HLA - B7抗体。同时，不同供体的细胞在针对HLA“主干”决定簇的单克隆抗体结合方面仅表现出非常小的差异。相对于总的HLA - A、 - B和 - C表达，淋巴细胞和血小板上HLA - A2的量相似。另一方面，与外周血淋巴细胞相比，血小板上HLA - B7的表达减少。
