Department of Bionano Technology, Gachon University, Bokjung-dong, Sujung-gu, Sungnam-si, Gyunggi-do 461-701, Republic of Korea.
J Biotechnol. 2013 Apr 15;164(4):543-8. doi: 10.1016/j.jbiotec.2012.12.017. Epub 2013 Jan 24.
We investigated the effect of arrayed nanostructures on the cell adhesion rate by forming nanopillars on a PMMA polymer surface, and demonstrated cell patterning tools for the polymer surface without biological or chemical reagents. Nanopillar arrayed structures with various heights (0, 50, 100, 150, and 200nm with 50nm of pitch size and 60nm of diameter) were formed on a PMMA surface by using nano-molding techniques with nanoporous AAO (anodic aluminum oxide) as a template. The nanopillar arrayed structures provide negative effects on the cell adhesion on the non-treated PMMA (moderate hydrophobic, ≥80° of contact angle), whereas slightly positive and no effects were shown by nanopillar structures on plasma (hydrophilic, ≤20°) and silane-treated PMMA (moderate, 40-70°), respectively. The microstructure on the polymer surface showed a 20% positive effect on the cell adhesion rate. As a result, nano or micro patterning structures could control the cell adhesion rate (15-120%) and it enabled the formation of closed cell patterns on the PMMA surface without chemical or biological surface treatments.
我们通过在 PMMA 聚合物表面形成纳米柱来研究阵列纳米结构对细胞黏附率的影响，并展示了无需生物或化学试剂的聚合物表面的细胞图案化工具。通过使用纳米压印技术和纳米多孔 AAO（阳极氧化铝）作为模板，在 PMMA 表面形成了具有不同高度（0、50、100、150 和 200nm，间距尺寸为 50nm，直径为 60nm）的纳米柱阵列结构。纳米柱阵列结构对未经处理的 PMMA（中等疏水性，≥80°接触角）上的细胞黏附产生负面影响，而等离子体（亲水性，≤20°）和硅烷处理的 PMMA（中等疏水性，40-70°）上的纳米柱结构则分别显示出轻微的正影响和无影响。聚合物表面的微观结构对细胞黏附率有 20%的积极影响。结果表明，纳米或微图案化结构可以控制细胞黏附率（15-120%），并且可以在没有化学或生物表面处理的情况下在 PMMA 表面形成封闭的细胞图案。
