Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Unidad Asociada de Entomología UJI-IVIA-CIB CSIC, Moncada, Spain.
Pest Manag Sci. 2013 Jul;69(7):857-64. doi: 10.1002/ps.3448. Epub 2013 Jan 28.
The success of an area-wide sterile insect technique (SIT) programme against Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) relies on the mating success of sterile males in the field. Limited information is available about the effectiveness of sterile males in achieving mates with wild females and how these matings contribute to reducing wild populations. To this end, firstly a mating competition test was performed in the laboratory with different release ratios (1:1:0, 1:1:1, 1:1:5, 1:1:10 and 1:1:20 for wild females:wild males:sterile VIENNA-8 males respectively) and different host fruit. Secondly, the same release ratios were evaluated under semi-natural conditions on caged trees and on sentinel host.
By means of molecular markers, VIENNA-8 male sperm was positively detected in those females exposed to the male ratios 1:5, 1:10 and 1:20 in the laboratory. In the field test, sterile VIENNA-8 male matings and the C. capitata progeny on apples were positively correlated with the ratio of sterile males released and with the percentage of sterile matings respectively.
These results confirm the validity of using the molecular detection of VIENNA-8 male sperm to predict the C. capitata population under semi-natural conditions. Implications of these results in measuring the efficacy of an SIT programme are discussed.
区域范围的不育昆虫技术（SIT）计划对地中海实蝇（Wiedemann）（双翅目：瘿蚊科）的成功依赖于不育雄虫在野外的交配成功率。关于不育雄虫与野生雌虫交配的有效性以及这些交配如何有助于减少野生种群的信息有限。为此，首先在实验室中进行了不同释放比例（野生雌虫：野生雄虫：Vienna-8 不育雄虫分别为 1:1:0、1:1:1、1:1:5、1:1:10 和 1:1:20）和不同宿主果实的交配竞争测试。其次，在半自然条件下，在笼养树上和哨兵宿主上评估了相同的释放比例。
通过分子标记，在实验室中，暴露于雄性比例为 1:5、1:10 和 1:20 的雌性中，检测到了 VIENNA-8 雄虫的精子。在田间试验中，不育 VIENNA-8 雄虫与苹果上的地中海实蝇后代与释放的不育雄虫比例以及不育交配的百分比呈正相关。
这些结果证实了使用 VIENNA-8 雄虫精子的分子检测来预测半自然条件下地中海实蝇种群的有效性。讨论了这些结果在衡量 SIT 计划效果方面的意义。
