Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
J Biomed Mater Res A. 2013 Aug;101(8):2295-305. doi: 10.1002/jbm.a.34523. Epub 2013 Jan 27.
3D interconnected porous scaffolds of HA and HA with various additions of SiO2 were fabricated using a polymeric template technique, to make bioceramic scaffolds consisting of macrostructures of the interconnected macropores. Three different sizes of the polyurethane template were used in the fabrication process to form different size interconnected macropores, to study the effect of pore size on human osteoblast cell viability. The template used allowed fabrication of scaffolds with pore sizes of 45, 60, and 75 ppi, respectively. Scanning microscopy was used extensively to observe the microstructure of the sintered samples and the characteristics of cells growing on the HA surfaces of the interconnected macropores. It has been clearly demonstrated that the SiO2 addition has influenced both the phase transformation of HA to TCP (β-TCP and α-TCP) and also affected the human osteoblast cell viability grown on these scaffolds.
采用高分子模板技术制备了具有不同 SiO2 添加量的 HA 和 HA 的 3D 互穿多孔支架，以制造由互穿大孔宏观结构组成的生物陶瓷支架。在制备过程中使用了三种不同尺寸的聚氨酯模板来形成不同尺寸的互穿大孔，以研究孔径对人成骨细胞活力的影响。所使用的模板允许制造孔径分别为 45、60 和 75 ppi 的支架。扫描显微镜被广泛用于观察烧结样品的微观结构和在互穿大孔的 HA 表面上生长的细胞的特征。已经清楚地表明，SiO2 的添加不仅影响了 HA 向 TCP（β-TCP 和 α-TCP）的相转变，而且还影响了在这些支架上生长的人成骨细胞活力。
