Department of Chemistry and Research Institute for Basic Sciences, Kyung Hee University, Seoul 130-701, Republic of Korea.
Biosens Bioelectron. 2013 May 15;43:362-5. doi: 10.1016/j.bios.2012.12.050. Epub 2013 Jan 3.
Aptamer-based biochips for selective cell detection and quantitation in combination of the recent biochip technology and the conventional cell staining methods are described. Using a model system comprising HER2- or PSMA-positive cells as the analytes and single-stranded RNA aptamers specific for HER2 or PSMA as immobilized ligands on chips, we could demonstrate that aptamers were equivalent or superior to antibodies in terms of specificity and sensitivity, respectively. In particular, our PSMA-specific sensor was found to have the characteristics of good stability, reproducibility and reusability, with detection limit as low as 10(3) LNCaP cells. In conclusion, we could show the suitability of nucleic acid aptamers as low molecular weight receptors on biochips for sensitive and specific cell detection and quantitation for future diagnostics development.
基于适体的生物芯片用于选择性细胞检测和定量,结合了最近的生物芯片技术和传统的细胞染色方法。使用包含 HER2 或 PSMA 阳性细胞作为分析物的模型系统,以及针对 HER2 或 PSMA 的单链 RNA 适体作为芯片上的固定配体,我们证明了适体在特异性和灵敏度方面分别与抗体相当或优于抗体。特别是,我们的 PSMA 特异性传感器具有良好的稳定性、重现性和可重复性,检测限低至 10(3)LNCaP 细胞。总之,我们可以证明核酸适体作为生物芯片上的小分子受体用于敏感和特异性细胞检测和定量的适用性,用于未来的诊断开发。