基于分子信标和氧化石墨烯的荧光生物传感器用于 Cu(2+)检测。
A molecular beacon and graphene oxide-based fluorescent biosensor for Cu(2+) detection.
机构信息
Department of Mechanical Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
出版信息
Biosens Bioelectron. 2013 May 15;43:379-83. doi: 10.1016/j.bios.2012.12.056. Epub 2013 Jan 4.
In this work, we report a "turn-on" fluorescent strategy for the direct detection of Cu(2+) in solutions using molecular beacons (MBs) and graphene oxide (GO). MBs are special single-stranded DNA and carry fluorescence sources. GO is a new nanomaterial having remarkable physical properties. In the sensing system, GO was used as an efficient fluorescence quencher upon the adsorption of MBs, which reduced the background signal and made the detection method highly sensitive. In the presence of Cu(2+), the MBs were cut into short pieces and released by the GO, leading to fluorescence restoration. The detection limit of the sensing strategy was ∼50nM, which is sufficiently sensitive for practical applications. The sensing method also exhibited high selectivity in testing samples containing other metal ions. The application of the method for drinking water is demonstrated.
在这项工作中，我们报告了一种使用分子信标 (MBs) 和氧化石墨烯 (GO) 的“开启”荧光策略，用于直接检测溶液中的 Cu(2+)。MBs 是特殊的单链 DNA，携带荧光源。GO 是一种具有显著物理性质的新型纳米材料。在传感系统中，GO 作为一种有效的荧光猝灭剂，吸附 MBs 后，降低了背景信号，使检测方法具有高度的灵敏度。在存在 Cu(2+)的情况下，MBs 被切成小块并被 GO 释放，导致荧光恢复。传感策略的检测限约为 50nM，对于实际应用来说已经足够灵敏。该传感方法在检测含有其他金属离子的样品时也表现出高选择性。该方法在饮用水中的应用得到了证明。
Zotero 插件