Institute of Laboratory Animal Science, Chinese Academy of Medical Science (CAMS) & Comparative Medical Center, Peking Union Medical College (PUMC), Beijing, China.
Neuroscience. 2013 May 15;238:371-80. doi: 10.1016/j.neuroscience.2013.01.034. Epub 2013 Jan 26.
Insulin plays diverse roles, including learning and memory, in the central nervous system. SIRT1 has been reported to be involved in the processes of normal learning, memory, and synaptic plasticity. However, whether insulin is directly involved in regulating SIRT1 expression in neurons or whether it affects synapses remains largely unknown. Here, we show that insulin promotes neurite outgrowth and increases SIRT1 expression in SH-SY5Y cells. LY294002, a phosphatidylinositol 3-kinase inhibitor, inhibited the expression of insulin-induced increases in SIRT1. Conversely, the downregulation of SIRT1 using a SIRT1 inhibitor and SIRT1-siRNA resulted in a significant reduction in the length of neurite outgrowth. Taken together, these results suggest that the regulation of SIRT1 by insulin is important for the neurite outgrowth of neuroblastoma cells.
胰岛素在中枢神经系统中发挥多种作用，包括学习和记忆。SIRT1 已被报道参与正常学习、记忆和突触可塑性过程。然而，胰岛素是否直接参与调节神经元中的 SIRT1 表达，或者它是否影响突触，在很大程度上仍然未知。在这里，我们表明胰岛素促进 SH-SY5Y 细胞的神经突生长并增加 SIRT1 的表达。PI3K 抑制剂 LY294002 抑制胰岛素诱导的 SIRT1 表达增加。相反，使用 SIRT1 抑制剂和 SIRT1-siRNA 下调 SIRT1 会导致神经突生长长度显著减少。总之，这些结果表明胰岛素对 SIRT1 的调节对神经母细胞瘤细胞的神经突生长很重要。
