Chemistry Department, Faculty of Science, Mansoura University, Egypt.
Int J Biol Macromol. 2013 Apr;55:207-13. doi: 10.1016/j.ijbiomac.2013.01.020. Epub 2013 Jan 25.
An enantioselective S-mandelic acid (S-MA) imprinted chitosan (SMIC) was prepared by cross-linking of chitosan using formaldehyde cross-linker, in the presence of S-MA as an imprint template molecule and 0.5% acetic acid solution as a solvent. Non-imprinted cross-linked chitosan (NIC) as control was also prepared by the same procedure in absence of template molecules. The surface morphology of both SMIC and NIC were examined by scanning electron microscope (SEM). SMIC particles were applied to determine the optimum operational condition for S-MA separation from dilute aqueous solution. In adsorption step, optimum pH and retention time were 3.5 and 60 min, while corresponding values in extraction step were 1 and 40 min, respectively. Also, the adsorption isotherms indicated that the maximum adsorption capacities of S- and R-MA on SMIC were 100 ± 0.5 and 64 ± 0.8 mg/g, respectively, while in the case of NIC, both R- and S-MA present the same maximum adsorption.
采用甲醛交联剂，以 S-扁桃酸（S-MA）为模板分子，0.5%乙酸溶液为溶剂，通过交联壳聚糖制备了对映体选择性 S-扁桃酸印迹壳聚糖（SMIC）。也通过相同的程序在没有模板分子的情况下制备了非印迹交联壳聚糖（NIC）作为对照。通过扫描电子显微镜（SEM）检查了 SMIC 和 NIC 的表面形态。SMIC 颗粒用于确定从稀水溶液中分离 S-MA 的最佳操作条件。在吸附步骤中，最佳 pH 值和保留时间分别为 3.5 和 60 min，而在萃取步骤中，相应的值分别为 1 和 40 min。此外，吸附等温线表明，SMIC 对 S-和 R-MA 的最大吸附容量分别为 100 ± 0.5 和 64 ± 0.8 mg/g，而在 NIC 的情况下，R-和 S-MA 具有相同的最大吸附。
