State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P.R. China.
Analyst. 2013 Jun 21;138(12):3433-43. doi: 10.1039/c3an36930g. Epub 2013 May 3.
In this paper, the molecularly imprinted polymers (MIPs) were prepared to recognize the template ovalbumin. The graft copolymerization was conducted based on chitosan (CS) and several types of functional monomers, while choosing N,N-methylenebisacrylamide as the cross-linking agent. The influence of the synthesis temperature was investigated. The effect of different kinds of functional monomers was compared and optimized. The properties of the obtained gels were characterized by using the FT-IR spectrometer, field emission scanning electron microscope (FESEM) and Zeta-meter. The adsorption isotherms of the imprinted chitosan gels were determined and well fitted by a Langmuir model. The maximum theoretical adsorption capacities (Q(max)) was determined to be 9.74 mg g(-1) for the MIP (CS-g-AAm) and 22.94 mg g(-1) for the MIP (CS-g-MAA). Several types of reference protein with different molecular weights and isoelectric points were selected to examine the selectivity of the chitosan based gels. The results implied that the charge effect and the shape memory were the critical factors affecting the rebinding process. Finally, the selectivity for the protein mixture adsorption was evaluated by the high efficiency liquid chromatography (HPLC) analysis choosing lysozyme as the competitive protein. These results demonstrate the potential selectivity of the prepared chitosan gels.
本文制备了分子印迹聚合物（MIPs）以识别模板卵清蛋白。接枝共聚反应是基于壳聚糖（CS）和几种类型的功能单体进行的，而选择 N,N-亚甲基双丙烯酰胺作为交联剂。考察了合成温度的影响。比较和优化了不同类型的功能单体的效果。使用傅里叶变换红外光谱仪（FT-IR）、场发射扫描电子显微镜（FESEM）和 Zeta 计对获得的凝胶的性质进行了表征。测定了印迹壳聚糖凝胶的吸附等温线，并通过 Langmuir 模型很好地拟合。MIP（CS-g-AAm）的最大理论吸附容量（Q(max)）确定为 9.74mg g(-1)，MIP（CS-g-MAA）的最大理论吸附容量（Q(max)）确定为 22.94mg g(-1)。选择了几种具有不同分子量和等电点的参考蛋白质来检查基于壳聚糖的凝胶的选择性。结果表明，电荷效应和形状记忆是影响复配过程的关键因素。最后，通过高效液相色谱（HPLC）分析选择溶菌酶作为竞争蛋白质来评估蛋白质混合物吸附的选择性。这些结果表明了所制备的壳聚糖凝胶的潜在选择性。
