• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Effect of Trastuzumab on Notch-1 Signaling Pathway in Breast Cancer SK-BR3 Cells.曲妥珠单抗对乳腺癌 SK-BR3 细胞 Notch-1 信号通路的影响。
Chin J Cancer Res. 2012 Sep;24(3):213-9. doi: 10.1007/s11670-012-0213-9.
2
Antitumoral actions of the anti-obesity drug orlistat (XenicalTM) in breast cancer cells: blockade of cell cycle progression, promotion of apoptotic cell death and PEA3-mediated transcriptional repression of Her2/neu (erbB-2) oncogene.抗肥胖药物奥利司他（赛尼可TM）对乳腺癌细胞的抗肿瘤作用：阻断细胞周期进程、促进凋亡性细胞死亡以及PEA3介导的Her2/neu（erbB-2）癌基因转录抑制。
Ann Oncol. 2005 Aug;16(8):1253-67. doi: 10.1093/annonc/mdi239. Epub 2005 May 3.
3
MTDH mediates trastuzumab resistance in HER2 positive breast cancer by decreasing PTEN expression through an NFκB-dependent pathway.MTDH通过依赖NFκB的途径降低PTEN表达，介导HER2阳性乳腺癌对曲妥珠单抗的耐药性。
BMC Cancer. 2014 Nov 24;14:869. doi: 10.1186/1471-2407-14-869.
4
In vitro comparative evaluation of trastuzumab (Herceptin) combined with paclitaxel (Taxol) or docetaxel (Taxotere) in HER2-expressing human breast cancer cell lines.曲妥珠单抗（赫赛汀）联合紫杉醇（泰素）或多西他赛（泰索帝）在HER2过表达人乳腺癌细胞系中的体外比较评估
Ann Oncol. 2002 Nov;13(11):1743-8. doi: 10.1093/annonc/mdf263.
5
Imaging and monitoring HER2 expression in breast cancer during trastuzumab therapy with a peptide probe Tc-HYNIC-H10F.使用肽探针Tc-HYNIC-H10F在曲妥珠单抗治疗期间对乳腺癌中的HER2表达进行成像和监测。
Eur J Nucl Med Mol Imaging. 2020 Oct;47(11):2613-2623. doi: 10.1007/s00259-020-04754-6. Epub 2020 Mar 13.
6
Novel signaling molecules implicated in tumor-associated fatty acid synthase-dependent breast cancer cell proliferation and survival: Role of exogenous dietary fatty acids, p53-p21WAF1/CIP1, ERK1/2 MAPK, p27KIP1, BRCA1, and NF-kappaB.与肿瘤相关脂肪酸合酶依赖性乳腺癌细胞增殖和存活相关的新型信号分子：外源性膳食脂肪酸、p53-p21WAF1/CIP1、ERK1/2 MAPK、p27KIP1、BRCA1和NF-κB的作用
Int J Oncol. 2004 Mar;24(3):591-608.
7
[Isolation of CD44+/CD24 -/low and side population cells from MDA-MB-453 cells and the analysis of their activation of Wnt and Notch pathway].从MDA-MB-453细胞中分离CD44+/CD24 -/低表达细胞和侧群细胞及其Wnt和Notch信号通路激活分析
Beijing Da Xue Xue Bao Yi Xue Ban. 2008 Oct 18;40(5):471-5.
8
[Molecular mechanisms of resistance to phosphatidyl inositol 3-kinase inhibitors in triple-negative breast cancer cells].三阴性乳腺癌细胞对磷脂酰肌醇3-激酶抑制剂耐药的分子机制
Zhonghua Zhong Liu Za Zhi. 2016 Aug;38(8):578-88. doi: 10.3760/cma.j.issn.0253-3766.2016.08.004.
9
Tumor-initiating cells of HER2-positive carcinoma cell lines express the highest oncoprotein levels and are sensitive to trastuzumab.HER2阳性癌细胞系的肿瘤起始细胞表达最高水平的癌蛋白，并且对曲妥珠单抗敏感。
Clin Cancer Res. 2009 Mar 15;15(6):2010-21. doi: 10.1158/1078-0432.CCR-08-1327. Epub 2009 Mar 10.
10
Local Radiation Treatment of HER2-Positive Breast Cancer Using Trastuzumab-Modified Gold Nanoparticles Labeled with Lu.使用镥标记的曲妥珠单抗修饰金纳米颗粒对HER2阳性乳腺癌进行局部放射治疗。
Pharm Res. 2017 Mar;34(3):579-590. doi: 10.1007/s11095-016-2082-2. Epub 2016 Dec 16.

引用本文的文献

1
Sensitization of MCF7 Cells with High Notch1 Activity by Cisplatin and Histone Deacetylase Inhibitors Applied Together.顺铂和组蛋白去乙酰化酶抑制剂联合应用对高 Notch1 活性 MCF7 细胞的致敏作用。
Int J Mol Sci. 2021 May 13;22(10):5184. doi: 10.3390/ijms22105184.
2
Circulating-free DNA Mutation Associated with Response of Targeted Therapy in Human Epidermal Growth Factor Receptor 2-positive Metastatic Breast Cancer.循环游离DNA突变与人类表皮生长因子受体2阳性转移性乳腺癌靶向治疗反应的相关性
Chin Med J (Engl). 2017 Mar 5;130(5):522-529. doi: 10.4103/0366-6999.200542.
3
miR-217 and CAGE form feedback loop and regulates the response to anti-cancer drugs through EGFR and HER2.微小RNA-217与癌症相关基因（CAGE）形成反馈回路，并通过表皮生长因子受体（EGFR）和人表皮生长因子受体2（HER2）调节对抗癌药物的反应。
Oncotarget. 2016 Mar 1;7(9):10297-321. doi: 10.18632/oncotarget.7185.
4
Combination therapy of RY10-4 with the γ-secretase inhibitor DAPT shows promise in treating HER2-amplified breast cancer.RY10-4与γ-分泌酶抑制剂DAPT联合治疗在HER2扩增型乳腺癌治疗中显示出前景。
Oncotarget. 2016 Jan 26;7(4):4142-54. doi: 10.18632/oncotarget.6769.
5
The Therapeutic Challenge of Targeting HER2 in Endometrial Cancer.子宫内膜癌中靶向HER2的治疗挑战
Oncologist. 2015 Sep;20(9):1058-68. doi: 10.1634/theoncologist.2015-0149. Epub 2015 Jun 22.
6
Differential peripheral blood gene expression profile based on Her2 expression on primary tumors of breast cancer patients.基于乳腺癌患者原发肿瘤中Her2表达的外周血基因差异表达谱。
PLoS One. 2014 Jul 28;9(7):e102764. doi: 10.1371/journal.pone.0102764. eCollection 2014.

本文引用的文献

1
Notch is an essential upstream regulator of NF-κB and is relevant for survival of Hodgkin and Reed-Sternberg cells.Notch 是 NF-κB 的重要上游调节因子，与霍奇金和 Reed-Sternberg 细胞的存活有关。
Leukemia. 2012 Apr;26(4):806-13. doi: 10.1038/leu.2011.265. Epub 2011 Sep 27.
2
Potent anti-proliferative effects of metformin on trastuzumab-resistant breast cancer cells via inhibition of erbB2/IGF-1 receptor interactions.二甲双胍通过抑制 erbB2/IGF-1 受体相互作用对曲妥珠单抗耐药乳腺癌细胞的强效抗增殖作用。
Cell Cycle. 2011 Sep 1;10(17):2959-66. doi: 10.4161/cc.10.17.16359.
3
Role of Notch and its oncogenic signaling crosstalk in breast cancer.Notch及其致癌信号串扰在乳腺癌中的作用。
Biochim Biophys Acta. 2011 Apr;1815(2):197-213. doi: 10.1016/j.bbcan.2010.12.002. Epub 2010 Dec 28.
4
Pro-apoptotic and anti-proliferative effects of mitofusin-2 via Bax signaling in hepatocellular carcinoma cells.线粒体融合蛋白 2 通过 Bax 信号通路在肝癌细胞中的促凋亡和抗增殖作用。
Med Oncol. 2012 Mar;29(1):70-6. doi: 10.1007/s12032-010-9779-6. Epub 2010 Dec 29.
5
Notch induces cyclin-D1-dependent proliferation during a specific temporal window of neural differentiation in ES cells.Notch 在胚胎干细胞的神经分化过程中特定的时间窗内诱导 cyclin-D1 依赖性增殖。
Dev Biol. 2010 Dec 15;348(2):153-66. doi: 10.1016/j.ydbio.2010.09.018. Epub 2010 Sep 29.
6
PTEN, PIK3CA, p-AKT, and p-p70S6K status: association with trastuzumab response and survival in patients with HER2-positive metastatic breast cancer.PTEN、PIK3CA、p-AKT 和 p-p70S6K 状态：与曲妥珠单抗治疗 HER2 阳性转移性乳腺癌患者的反应和生存的相关性。
Am J Pathol. 2010 Oct;177(4):1647-56. doi: 10.2353/ajpath.2010.090885. Epub 2010 Sep 2.
7
Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer (ToGA): a phase 3, open-label, randomised controlled trial.曲妥珠单抗联合化疗与单纯化疗治疗 HER2 阳性晚期胃或胃食管交界腺癌（ToGA）：一项开放标签、随机对照的 3 期临床试验。
Lancet. 2010 Aug 28;376(9742):687-97. doi: 10.1016/S0140-6736(10)61121-X. Epub 2010 Aug 19.
8
Notch-1 and notch-4 receptors as prognostic markers in breast cancer.Notch-1和Notch-4受体作为乳腺癌的预后标志物
Int J Surg Pathol. 2011 Oct;19(5):607-13. doi: 10.1177/1066896910362080. Epub 2010 May 5.
9
Mechanistic and signaling analysis of Muc4-ErbB2 signaling module: new insights into the mechanism of ligand-independent ErbB2 activity.Muc4-ErbB2 信号模块的机制和信号分析：对配体非依赖性 ErbB2 活性机制的新认识。
J Cell Physiol. 2010 Sep;224(3):649-57. doi: 10.1002/jcp.22163.
10
Expression of vascular notch ligand delta-like 4 and inflammatory markers in breast cancer.血管结合蛋白 delta-like 4 及其在乳腺癌中炎症标志物的表达。
Am J Pathol. 2010 Apr;176(4):2019-28. doi: 10.2353/ajpath.2010.090908. Epub 2010 Feb 18.

曲妥珠单抗对乳腺癌 SK-BR3 细胞 Notch-1 信号通路的影响。

Effect of Trastuzumab on Notch-1 Signaling Pathway in Breast Cancer SK-BR3 Cells.

机构信息

Department of Pathophysiology, Laboratory for Stem Cell and Tissue Engineering, Chongqing Medical University, Chongqing 400016, China.

出版信息

Chin J Cancer Res. 2012 Sep;24(3):213-9. doi: 10.1007/s11670-012-0213-9.

DOI:10.1007/s11670-012-0213-9
PMID:23358465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3555277/
Abstract

OBJECTIVE

To investigate the effects and mechanisms of trastuzumab on Notch-1 pathway in breast cancer cells, recognizing the significance of Notch-1 signaling pathway in trastuzumab resistance.

METHODS

Immunocytochemistry staining and Western blotting were employed to justify the expression of Notch-1 protein in HER2-overexpressing SK-BR3 cells and HER2-non-overexpressing breast cancer MDA-MB-231 cells. Western blotting and reverse transcription PCR (RT-PCR) were used to detect the activated Notch-1 and Notch-1 target gene HES-1 mRNA expression after SK-BR3 cells were treated with trastuzumab. Double immunofluorescence staining and co-immunoprecipitation were used to analyze the relationship of Notch-1 and HER2 proteins.

RESULTS

The level of Notch-1 nuclear localization and activated Notch-1 proteins in HER2-overexpressing cells were significantly lower than in HER2-non-overexpressing cells (P<0.01), and the expressions of activated Notch-1 and HES-1 mRNA were obviously increased after trastuzumab treatment (P<0.05), but HER2 expression did not change significantly for trastuzumab treating (P>0.05). Moreover, Notch-1 was discovered to co-localize and interact with HER2 in SK-BR3 cells.

CONCLUSION

Overexpression of HER2 decreased Notch-1 activity by the formation of a HER2-Notch1 complex, and trastuzumab can restore the activity of Notch-1 signaling pathway, which could be associated with cell resistance to trastuzumab.

摘要

目的

研究曲妥珠单抗对乳腺癌细胞 Notch-1 通路的作用及其机制，认识 Notch-1 信号通路在曲妥珠单抗耐药中的意义。

方法

采用免疫细胞化学染色和 Western blot 法验证曲妥珠单抗耐药的人表皮生长因子受体 2（HER2）过表达 SK-BR3 细胞和 HER2 非过表达乳腺癌 MDA-MB-231 细胞中 Notch-1 蛋白的表达。Western blot 和逆转录聚合酶链反应（RT-PCR）检测 SK-BR3 细胞经曲妥珠单抗处理后激活的 Notch-1 及其靶基因 HES-1mRNA 的表达。双免疫荧光染色和免疫共沉淀分析 Notch-1 与 HER2 蛋白的关系。

结果

HER2 过表达细胞 Notch-1 核内定位和激活型 Notch-1 蛋白水平明显低于 HER2 非过表达细胞（P<0.01），曲妥珠单抗处理后激活型 Notch-1 和 HES-1mRNA 的表达明显增加（P<0.05），但 HER2 表达无明显变化（P>0.05）。此外，在 SK-BR3 细胞中发现 Notch-1 与 HER2 共定位并相互作用。

结论

HER2 的过表达通过形成 HER2-Notch1 复合物降低 Notch-1 的活性，曲妥珠单抗可恢复 Notch-1 信号通路的活性，这可能与细胞对曲妥珠单抗的耐药性有关。