评估 XAF1 作为鉴别肝细胞癌与非肿瘤性肝组织的生物标志物。
Assessment of XAF1 as A Biomarker to Differentiate Hepatocellular Carcinoma from Nonneoplastic Liver Tissues.
机构信息
Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai 201620, China.
出版信息
Chin J Cancer Res. 2012 Sep;24(3):201-6. doi: 10.1007/s11670-012-0201-0.
OBJECTIVE
XIAP-associated factor 1 (XAF1) expression has been shown to be related with apoptosis in hepatocellular carcinoma (HCC). However, the correlation of XAF1 expression with HCC tumor grade has not been intensively assessed. XIAP-associated factor-1 (XAF1) is an important apoptosis inducer in human HCC. The aim of this study is to determine the correlation between XAF1 expression and HCC histopathological grades.
METHODS
The mRNA levels of XAF1 in 24 paired HCC-nonneoplastic specimens were quantified by real-time reverse transcription PCR (RT-PCR). Protein levels of XAF1 in 110 paired HCC-noncancer tissues were investigated by immunostaining specimens on a tissue microarray (TMA). Correlations between XAF1 mRNA levels or protein expression and clinicopathological features were assessed by statistical analysis.
RESULTS
Both XAF1 mRNA and protein were significantly under-expressed in HCC tissues compared to their non-neoplastic counterparts. No significant relationship was found between XAF1 mRNA or protein expression and histological tumor grade.
CONCLUSION
All these data suggest that XAF1 is a potential biomarker for differentiating HCC with noncancerous tissues.
目的
XIAP 相关因子 1（XAF1）的表达与肝癌（HCC）中的细胞凋亡有关。然而，XAF1 表达与 HCC 肿瘤分级的相关性尚未得到深入评估。XAF1 是人类 HCC 中一种重要的凋亡诱导因子。本研究旨在确定 XAF1 表达与 HCC 组织病理学分级之间的相关性。
方法
通过实时逆转录 PCR（RT-PCR）定量检测 24 对 HCC-非肿瘤标本中 XAF1 的 mRNA 水平。通过免疫组织化学染色对组织微阵列（TMA）上的 110 对 HCC-非癌组织中的 XAF1 蛋白水平进行检测。通过统计分析评估 XAF1 mRNA 水平或蛋白表达与临床病理特征之间的相关性。
结果
与非肿瘤组织相比，XAF1 mRNA 和蛋白在 HCC 组织中均显著低表达。XAF1 mRNA 或蛋白表达与组织学肿瘤分级之间无显著关系。
结论
所有这些数据表明，XAF1 是区分 HCC 与非癌组织的潜在生物标志物。
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