Department of Reparative Materials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto-city, Kyoto, Japan.
J Biomed Mater Res B Appl Biomater. 2013 May;101(4):656-62. doi: 10.1002/jbm.b.32869. Epub 2013 Jan 29.
Endovascular treatment of intracranial aneurysms with detachable coils has been accepted widely. Problems of coil compaction, recanalization and rare endothelialization at the aneurysm orifice are not yet solved. We investigated the efficacy of a simvastatin coating applied without any additional matrix to coils to accelerate thrombus organization in the cavity in a rat model of aneurysm. Twelve metal coils coated with simvastatin and 12 bare coils were inserted into the ligated external carotid arterial (ECA) sacs of rats. The ECA sacs were removed 2 or 4 weeks after the coils were implanted and examined by histology and immunohistochemical assay. The organized areas in the ECA sacs in the simvastatin group (73.6 ± 19.4%, 2 wk; 83.4 ± 11.1%, 4 wk) was significantly higher p = 0.003, 2 wk; p = 0.0004, 4 wk than the bare metal group at 2 and 4 weeks (20.5 ± 10.7%, 2 wk, p < 0.003; 37.4 ± 20.6%, 4 wk, p < 0.0004). Organized tissues that formed around the coils coated with simvastatin were characterized by an accumulation of cells positive for αSMA and collagen connective matrix. Tissues also were accompanied by marked formation of endothelium at the orifice of the ECA sac. We suggest that coating coils with simvastatin effectively accelerated organization within the aneurysms and endothelialization over the coil.
颅内动脉瘤的可解脱弹簧圈血管内治疗已被广泛接受。然而，线圈压实、再通和动脉瘤口罕见内皮化等问题仍未得到解决。我们研究了在大鼠动脉瘤模型中，不使用任何额外基质将辛伐他汀涂层应用于线圈以加速腔内血栓形成的效果。将 12 个涂有辛伐他汀的金属线圈和 12 个裸线圈插入结扎的颈外动脉（ECA）囊中。在植入线圈后 2 或 4 周取出 ECA 囊，并通过组织学和免疫组织化学检测进行检查。辛伐他汀组 ECA 囊中的组织化区域（2 周时为 73.6 ± 19.4%，4 周时为 83.4 ± 11.1%）明显高于裸金属组（2 周时为 20.5 ± 10.7%，p < 0.003；4 周时为 37.4 ± 20.6%，p < 0.0004）。用辛伐他汀涂覆的线圈周围形成的组织化组织表现为积聚αSMA 和胶原结缔基质阳性的细胞。组织还伴有 ECA 囊口明显的内皮形成。我们认为，用辛伐他汀涂覆线圈可有效加速动脉瘤内的组织化和线圈上的内皮化。
